Application of L-Arginine as nitric oxide inducer and Indomethacin as prostaglandin inhibitor in Balb/c mice infected with Leishmania major MRHO/IR/75/ER

authors:

avatar Fatemeh Ghasemi 1 , * , avatar Hossein Nahrevanian 2

Researcher, AJA University of Medical Science, Department of Biochemistry, Tehran, Iran, Andorra
Resercher, Department of Parasitology, Pasteur Institute of Iran, Tehran, Iran, Andorra

how to cite: Ghasemi F, Nahrevanian H. Application of L-Arginine as nitric oxide inducer and Indomethacin as prostaglandin inhibitor in Balb/c mice infected with Leishmania major MRHO/IR/75/ER. Ann Mil Health Sci Res. 2013;11(1):e66701. 

Abstract

Background: Leishmaniasis is one of the health problems in. This study is designed to inhibit PG production by Indomethacin and induce NO by L- Arginine precursor in L. major infected Balb/c mice.
Materials and methods: This was an experimental study. Animals, Male inbred Balb/c mice were used in this study. The total number of animals used in this experiment was 48 Balb/c mice, divided into 6 groups (n =8 mice/group) including Group 1 (naive), Group 2 (L. major + 0.4% Ethanol), Group 3 (L. major + Indomethacin), Group 4 (L. major + DW), Group 5 (L. major + L- Arginine) and Group 6 (L. major + Indomethacin + LArginine). In addition to serum, liver and spleen suspensions were investigated for NO induction by using Griess microassay. The data was analyzed by Analysis of Variances (ANOVA) and Student’s t-test using Graph Pad Prism Software.
Results: The results indicated that production of NO was inhibited in infected Balb/c mice by L. major as compared with naive animals (Group 1). INDO as inhibitor PG (Group 2) showed their ability to elevate RNI levels in infect animals. INDO showed anti-leishmanial activity, as these compounds reduced the lesion sizes (P<0.001). INDO as inhibitor PG (Group 3) showed ability to decrease PG levels in infect animals.
Conclusion: Our data may indicate a possible role for L-Arg and INDO as novel drug targets for the treatment of cutaneous leishmaniasis in mouse model.

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