E-Selectin Gene Polymorphisms in Iranian Chronic Hepatitis B Patients

authors:

avatar Mehrdad Hajilooi 1 , avatar Amir-Houshang Mohammad Alizadeh 2 , avatar Mitra Ranjbar 2 , avatar Farahnaz Fallahian 2 , avatar Seyed Moayed Alavian 3 , *

Department of Immunology, Hamedan University of Medical Sciences, Hamedan, IR.Iran
Department of Gastroenterology, Shaheed Beheshti University of Medical Sciences, Tehran, IR.Iran
Professor of Gastroenterology and Hepatology, Baqiyatallah Research Center for Gastroenterology and Liver Disease (BRCGL), Baqiyatallah University of Medical Sciences, [email protected], Tehran, IR.Iran

how to cite: Hajilooi M, Alizadeh A, Ranjbar M, Fallahian F, Alavian S. E-Selectin Gene Polymorphisms in Iranian Chronic Hepatitis B Patients. Hepat Mon.7(4): 211-216.

Abstract

Background and Aims: The aim of this study is to detect the substitutions Ser128Arg (A128C) and Leu554Phe (T554C) which responsible for E-selectin polymorphisms in patients with chronic hepatitis B and healthy controls. We investigated association of the Ser128Arg, Leu554Phe gene polymorphisms in the E-selectin gene as prototypical inflammatory molecules for susceptibility to chronic hepatitis B.

Methods: Sixty-three patients with chronic hepatitis B virus infection and 150 healthy subjects were recruited sequentially as they presented to clinic. Genomic DNA was isolated from anti-coagulated peripheral blood Buffy coat using Miller's salting-out method. The presence of the E-selectin gene polymorphisms was determined by using polymerase chain reaction amplification refractory mutation system (ARMS).

Results: Distribution of E-selectin 128 (A+C-, A+C+, A-C+) genotypes and E-selectin genotype 554 (C+T-, T+C-, C+T+) genotypes were not statistically significant different in chronic hepatitis B patients and controls by Chi-square test (P=0.41 & 0.96, respectively). Also, Two groups had not statistically significant difference in distribution of frequencies of allele 128 A by Chi-square test (P=0.41), 128 C (P=0.15), allele 554 C (P=0.85), and allele 554 T (P=0.76). Carrying allele 128 A (OR=0.587, 95% CI=0.162-2.124), 128 C (OR=1.526, 95% CI=0.849-2.745), 554 C (OR=1.245, 95% CI=0.128-12.089), and allele 554 T (OR=0.880, 95% CI=0.384-2.014) were not risk factors for susceptibility to chronic hepatitis B infection.

Conclusions: Carrying E-selectin gene polymorphisms of Ser128Arg and Leu554Phe are not risk factors for susceptibility to chronic hepatitis B.

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