The differences in gene expression profile induced by genotype 1b hepatitis C virus core isolated from liver tumor and adjacent non-tumoral tissue

authors:

avatar Xue-bing Yan 1 , * , avatar Zhi Chen 2 , avatar Christian Brechot 3

Department of Infectious Diseases, the First Affiliated Hospital of Xuzhou Medical College, yxbxuzhou@126.com, China
Institute of Infectious Diseases, the First Affiliated Hospital, College of Medical Science, Key Laboratory of Ministry Health, Zhejiang University, China
Inserm, France, University Paris sud, Centre Hepato-Biliaire, Hopital Paul Brousse, France
Hepatitis Monthly: Vol.11, issue 4; 255-262
article type: Research Article
received: August 7, 2010
accepted: December 4, 2010

how to cite: Yan X, Chen Z, Brechot C. The differences in gene expression profile induced by genotype 1b hepatitis C virus core isolated from liver tumor and adjacent non-tumoral tissue. Hepat Mon. 2011;11(4): 255-262. 

Abstract

Background: Core protein of the hepatitis C virus (HCV) has an important role in HCV self-replication, pathogenesis and carcinogenesis.
Objectives: To identify the effect of core proteins from different quasispecies of HCV genotype 1b expressed in a HepG2 cell line on human gene expression profiles.
Materials and Methods: Core protein eukrocytic expression plasmids (pEGFP-N1) containing different quasispecie core protein genes of genotypes 1b HCV derived from of HCV-related hepatocellular carcinoma (HCC) tumoral tissue (T) and non-tumoral tissue (NT) were constructed and then transfected to HepG2 cell line. The gene expression spectrum in the cell expression core proteins from T and NT were compared with those in the control by Affymetrix human genome HG-U 133 plus 2.0 microarray.
Results: Different gene expression profiles were acquired between HepG2 expressing core proteins derived from T and NT tissues. Both core proteins caused the modulation of several genes that are up/down-regulated as compared to control, including genes involved in oncogenesis, signal transduction, cell apoptosis, and cell growth cycle regulation. Surprisingly, only one gene-CSNK1A1-was up-regulated by both T and NT core variants.
Conclusions: Core proteins isolated from tumoral or non-tumoral nodules mediate expression of different cellular genes suggesting that variants isolated from different quasispecies may have different biological effects.
Keywords: Hepatitis C virus; Viral core proteins; Genotypes; Microarray analysis; Gene expression

  • Implication for health policy/practice/research/medical education:
    The study of the role of core protein of HCV is recommended for all basic scientists especially those involved in treatment of HCC.
  • Please cite this paper as:
    Yan XB, Chen Z, Brechot C. The differences in gene expression profile induced by genotype 1b hepatitis C virus core isolated from liver tumor and adjacent non-tumoral tissue. Hepat Mon. 2011;11(4):255-262.

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