Intraperitoneal Transplantation of Hepatocytes Embedded in Thermoreversible Gelation Polymer (Mebiol Gel) in Acute Liver Failure Rat Model

authors:

avatar Nyamathulla Parveen 1 , avatar Aleem Ahmed Khan 2 , * , avatar Subramani Baskar 3 , avatar Mohammad Aejaz Habeeb 1 , avatar Ravindra Babu 1 , avatar Samuel Abraham 3 , avatar Hiroshi Yoshioka 4 , avatar Yuichi Mori 4 , avatar Habibullah Chittoor Mohammad 1

Centre for Liver Research and Diagnostics, Deccan College of Medical Sciences, India
Centre for Liver Research and Diagnostics, Deccan College of Medical Sciences, aleem_a_khan@rediffmail.com, A.P., India
Nichi-In Centre for Regenerative Medicine, India
Advance Research Centre for Science and Engineering, Waseda University, Japan
Hepatitis Monthly: Vol.8, issue 4; 275-280
article type: Research Article
received: May 15, 2008
accepted: September 18, 2008

How To Cite Parveen N, Khan A, Baskar S, Habeeb M, Babu R, et al. Intraperitoneal Transplantation of Hepatocytes Embedded in Thermoreversible Gelation Polymer (Mebiol Gel) in Acute Liver Failure Rat Model. Hepat Mon. 2008;8(4): 275-280. 

Abstract

Orthotopic liver transplantation (OLT) is the only treatment that improves the survival rate in patients with acute liver failure (ALF). Hepatocytes are anchorage dependent and require a substratum for their long-term survival. Various substrata are being used to improve the function and survival of these hepatocytes, but cannot be used clinically as they are animal derived. This has been overcome by thermoreversible gelation polymer (TGP). In the present study, rat hepatocytes embedded in TGP, were transplanted intraperitoneally in acute liver failure rat model. Efficacy of the transplanted cells was studied by assessing biochemical parameters and histopathology at different time points. The ALF condition reverted to normal 15 days after cell transplantation. The transplanted animals survived 100%. Therefore this study suggests that the cells in TGP provide substratum to the transplanted cells for long term survival and could successfully provide liver support in severe ALF.

Full Text

Full text is available in PDF