Orthotopic liver transplantation (OLT) is the only treatment that improves the survival rate in patients with acute liver failure (ALF). Hepatocytes are anchorage dependent and require a substratum for their long-term survival. Various substrata are being used to improve the function and survival of these hepatocytes, but cannot be used clinically as they are animal derived. This has been overcome by thermoreversible gelation polymer (TGP). In the present study, rat hepatocytes embedded in TGP, were transplanted intraperitoneally in acute liver failure rat model. Efficacy of the transplanted cells was studied by assessing biochemical parameters and histopathology at different time points. The ALF condition reverted to normal 15 days after cell transplantation. The transplanted animals survived 100%. Therefore this study suggests that the cells in TGP provide substratum to the transplanted cells for long term survival and could successfully provide liver support in severe ALF.
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