The effect of desacetyluvaricin on the expression of TLR4 and P53 protein in Hepg 2.2.15

Bin Yu; Hai Bin He; Xian Lin Wu; Kang Li Liu; Guang Xiong Zhou; Guo Qiang Qian; Da Hong Ju; Xiao Yin Chen; Xiao Chen

Hepatitis Monthly

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The effect of desacetyluvaricin on the expression of TLR4 and P53 protein in Hepg 2.2.15

Author(s):

Bin Yu,

Hai Bin He¹,

Xian Lin Wu²,

Kang Li Liu²,

Guang Xiong Zhou³,

Guo Qiang Qian²,

Da Hong Ju⁴,

Xiao Yin Chen^#2,

Xiao Chen^#5,*

1Department of Gastroenterology, the People’s Hospital of Heshan, Guangdong, China

2Department of Traditional Chinese Medicine, Medicine College of Jinan University, Guangzhou, China

3Pharmaceutical College of Jinan University, Guangzhou, China

4Institute of Basic Theories of Traditional Chinese Medicine, China Academy of Traditional Chinese Medicine, Beijing, China

5Shusen Zheng, Department of Hepatobiliary and Pancreatic Surgery, First Affiliated Hospital, Hangzhou, China. Tel: +86- 57187236570, Fax: +86-57187236466. E-mail: shusenzheng@zju.edu.cn

# These authors have contributed equally.

Hepatitis Monthly:Vol. 11, issue 5; 364-367

Published online:May 31, 2011

Article type:Research Article

Received:Aug 29, 2010

Accepted:Dec 04, 2010

How to Cite:Yu B, He H B, Wu X L, Liu K L, Zhou G X, et al. The effect of desacetyluvaricin on the expression of TLR4 and P53 protein in Hepg 2.2.15.Hepat Mon.2011;11(5):364-367.

Abstract

Background: Previous studies suggest that annonaceous may cause permeability glycoprotein (P-gp) function to abate, leading to cell apoptosis. It has also been reported that annonaceous acetogenins affect hepatocellular carcinoma (HCC) cells in the G1 phase, leading to apoptosis. Desacetyluvaricin (Des), a new type of annonaceous acetogenin monomer, has a significant effect on HCC, with few side effects.
Objectives: To investigate the effect of Des on the expression of Toll-like receptor 4 (TLR4) and P53 protein in HCC.
Materials and Methods: HCC HepG2.2.15 cell was cultured by routine method. HepG2.2.15 cells were divided into three groups: control group, treated with Des and DDP (cisplatin) which were examined by immunofluorescence flow cytometry for expression of TLR4 and P53.
Results: TLR4 was expressed by more cells in the Des group than in the cisplatin or serum-only groups (71.94%, 42.64%, and 37.16%, respectively; Des vs.cisplatin: p < 0.05; Des vs. serum only: p < 0.05), with no difference between the cisplatin and serum-only groups (p > 0.05). P53 was expressed by more cells in the Des and cisplatin groups than in the serum-only group (32.6%, 31.5% and 3.3%, respectively; Des vs. serum only, p < 0.05; cisplatin vs. serum only, p < 0.05), with no difference between the Des and cisplatin groups (p > 0.05).
Conclusions: Des increases TLR4 and P53 expression in HCC cells. Improved immune recognition by the former effect and induction of apoptosis by the latter could be the mechanisms of Des's clinical effects on HCC.
Keywords: Desacetyluvaricin; TLR4 receptor; P53 tumor suppressor protein; Flow cytometry; Hepatocellular carcinoma

Keywords

Desacetyluvaricin

TLR4 receptor

P53 tumor suppressor protein

Flow cytometry

Hepatocellular carcinoma

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Author(s):

Bin Yu:[PubMed][Scholar]
Hai Bin He:[PubMed][Scholar]
Xian Lin Wu:[PubMed][Scholar]
Kang Li Liu:[PubMed][Scholar]
Guang Xiong Zhou:[PubMed][Scholar]
Guo Qiang Qian:[PubMed][Scholar]
Da Hong Ju:[PubMed][Scholar]
Xiao Yin Chen:[PubMed][Scholar]
Xiao Chen:[PubMed][Scholar]

Official Journal of Research Center for Gastroenterology and Liver Diseases

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The effect of desacetyluvaricin on the expression of TLR4 and P53 protein in Hepg 2.2.15

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