Investigation of Transforming Growth Factor-?1 Gene Polymorphisms Among Iranian Patients With Chronic Hepatitis C

authors:

avatar Sara Romani 1 , avatar Pedram Azimzadeh 2 , * , avatar Seyed Reza Mohebbi 1 , avatar Shabnam Kazemian 1 , avatar Shohreh Almasi 1 , avatar Hamed Naghoosi 1 , avatar Faramarz Derakhshan 1 , avatar Mohammad Reza Zali 1

Research Center for Gastroenterology and Liver Diseases, Shahid Beheshti University of Medical Sciences, IR Iran
Research Center for Gastroenterology and Liver Diseases, Shahid Beheshti University of Medical Sciences, srmohebbi@rigld.com, IR Iran
Hepatitis Monthly: Vol.11, issue 11; 901-906
article type: Research Article
received: July 12, 2011
accepted: September 24, 2011
DOI: https://doi.org/10.5812/kowsar.1735143X.776

how to cite: Romani S, Azimzadeh P, Mohebbi S, Kazemian S, Almasi S, et al. Investigation of Transforming Growth Factor-?1 Gene Polymorphisms Among Iranian Patients With Chronic Hepatitis C. Hepat Mon. 2011;11(11): 901-906. https://doi.org/10.5812/kowsar.1735143X.776.

Abstract

Background: Chronic hepatitis C infection is caused by the hepatitis C virus (HCV), and its clinical complications include liver cirrhosis, liver failure, and hepatocellular carcinoma. Transforming growth factor-β1 (TGF-β1) is an important cytokine in cell growth and differentiation, angiogenesis, extracellular matrix formation, immune response regulation, and cancer development and progression.
Objectives: The aim of this study was to investigate the relationship between single nucleotide polymorphisms (SNPs) in TGF-β1 and chronic HCV infection among patients referred to the Taleghani Hospital, Tehran, Iran between 2008 and 2010.
Patients and Methods: In this case-control study, samples were collected using a convenience sampling method. We genotyped 164 HCV patients and 169 healthy controls for 3 SNPs in the TGF-β1 gene (-509 promoter, codon 10, and codon 25). We determined the SNP genotypes by using polymerase chain reaction-restriction fragment length polymorphism (PCR-RFLP) method. To confirm the PCR-RFLP genotyping results, 10% of the samples were re-genotyped using a direct sequencing method.
Results: There were no significant differences in the allelic frequency distribution of SNPs at -509 C/T, +869 C/T, or +915 G/C between HCV patients and the healthy controls. Genotyping results for all three polymorphic sites were similar with no statistically significant differences between the groups.
Conclusions: Most of the Iranian patients (over 85%), both healthy controls and HCV patients, had the GG genotype at the +915 G/C position, resulting in a high level of TGF-β1 production. Therefore, we concluded that the SNPs investigated by us cannot be considered as prognostic factors for HCV infection in our population, despite being reported as prognostic markers in other populations. Moreover, there is a possibility that most of the population is susceptible to HCV infection.

 

  

Implication for health policy/practice/research/medical education:
Transforming growth factor beta-1 (TGF-β1) is a multifunctional cytokine that acts as a regulator of proliferation and cellular differentiation.Investigation of the association of single nucleotide polymorphisms (SNPs) in TGF-β1 gene sequence with increased or decreased individual’s susceptibility to chronic diseases such as hepatitis C infection could be helpful to find appropriate and
population specific genetic biomarkers.

Please cite this paper as:
Romani S, Azimzadeh P, Mohebbi SR, Kazemian S, Almasi S, Naghoosi H, et al. Investigation of Transforming Growth Factor–β1 Gene Polymorphisms Among Iranian Patients With Chronic Hepatitis C. Hepat Mon. 2011;11(11):901-6. DOI: 10.5812/kowsar.1735143X.776

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