Protective Effect of Green Tea Aqueous Extract on Acrylamide Induced Neurotoxicity

authors:

avatar Elahe Esmaeelpanah 1 , avatar Alireza Rahmatkhah 1 , avatar Narges Poormahmood 1 , avatar Bibi Marjan Razavi 2 , avatar Faezeh Vahdati Hasani 1 , avatar Hossein Hosseinzadeh 3 , *

School of Pharmacy, Mashhad University of Medical Sciences, Mashhad, IR Iran
Targeted Drug Delivery Research Center, Department of Pharmacodynamy and Toxicology, School of Pharmacy, Mashhad University of Medical Sciences, Mashhad, IR Iran
Pharmaceutical Research Center, Department of Pharmacodynamy and Toxicology, School of Pharmacy, Mashhad University of Medical Sciences, Mashhad, IR Iran

how to cite: Esmaeelpanah E, Rahmatkhah A, Poormahmood N, Razavi B M, Vahdati Hasani F, et al. Protective Effect of Green Tea Aqueous Extract on Acrylamide Induced Neurotoxicity. Jundishapur J Nat Pharm Prod. 2015;10(2):e18406. https://doi.org/10.17795/jjnpp-18406.

Abstract

Background:

Acrylamide (ACR) monomer is an effective neurotoxicant, which damages the central and peripheral nervous systems in humans and animals. Green tea, an infusion from the leaves of Camellia sinensis Theaceae, is a known antioxidant traditional medicine.

Objectives:

In this study, the protective effect of green tea aqueous extract (GTAE) was evaluated on ACR induced neurotoxicity.

Materials and Methods:

In our in vitro study, the effect of different concentrations of GTAE on ACR toxicity (IC50) in PC12 cells was evaluated using MTT assay. Moreover in another experiment, the effect of GTAE on neural toxicity induced by ACR was evaluated in rats.

Results:

Treatment with ACR (50 mg/kg via intraperitoneal injection for 11 days), induced severe gait abnormalities and significantly decreased body weight at the end of 11 days. Treatment with GTAE (6.25, 12.5, 25 and 50 mg/kg) reduced ACR-induced neurotoxicity, but the effect was only significant in a group which received GTAE at a dose of 12.5 mg/kg and ACR (P < 0.01). ACR decreased cell viability in PC12 cells used as an in vitro model. Pretreatment with GTAE (7.8 - 62.5 µg/mL) decreased ACR-induced cytotoxicity (P < 0.01 and P < 0.001, respectively).

Conclusions:

As green tea is an essential source of antioxidants such as flavonoids, suppression of reactive oxygen species (ROS) generation may be in part considered as the neuroprotective mechanism on ACR induced neurotoxicity.

1. Background

Acrylamide (ACR) is a low-molecular-weight vinylic compound used to produce polyacrylamides. Polymers are used in different industries like wastewater treatment, soil coagulation, dye synthesis and gel chromatography in laboratories. ACR monomer is a potent neurotoxin and animal carcinogen; however, the polymer is not toxic (1-3). ACR has been known as an occupational hazard for decades (4, 5). Moreover it has been found to form in fried and baked starchy foods during cooking (6). Evidence has shown that ACR is a potent neurotoxicant in both humans and animals. Low-level exposure to ACR causes skeletal muscle weakness, ataxia, myalgia and weight loss (7). Beside neurotoxicity, ACR can induce reproductive toxicity, genotoxicity and carcinogenicity (8-10). It was shown that ACR subchronic exposure could affect the expression of death-related proteins in the central and peripheral nervous systems (11). Moreover, ACR increased intracellular reactive oxygen species (ROS) in PC12 cells, which played an important role in ACR induced cytotoxicity (12). Growing evidence has shown that enhancement of lipid peroxidation and impairment of antioxidative capacity in the central and peripheral nervous systems are considered as mechanisms of ACR-induced neuropathy (13).

Recently, there has been increasing interest in potential human health benefits of natural compounds. Green tea is made up of leaves of the Camellia sinensis Theaceae. Many of the profitable properties of green tea are related to the activities of epigallocatechin gallate (EGCG), the major compound of green tea catechins (14).

Green tea and its constituents are widely evaluated for their pharmacological activities such as anti-cancer (15, 16), anti-obesity (17, 18), anti-atherosclerotic (19), anti-diabetic (20), hepatoprotective (21), anti-bacterial and anti-viral effects (14). Green tea and its components have shown antioxidant effects in different in vitro and in vivo studies (22, 23).

Antioxidants have been reported to protect neurons from neural loss by reducing ROS-mediated reactions (24-27).

2. Objectives

In the present study, possible protective effect of GTAE on ACR-induced neurotoxicity was evaluated in both in vivo and in PC12 cells as a suitable in vitro model for evaluation of neurotoxicity.

3. Materials and Methods

3.1. Chemicals

RPMI 1640 and FBS were purchased from Gibco. (4,5-dimethylthiazol-2-yl)-2,5-diphenyl tetrazolium (MTT) and ACR were obtained from Sigma (USA) and Merck (Germany).

3.2. Plant Materials and Preparation of GTAE

Green tea leaves (Place of origin: North of Iran) were collected. The leaves were powdered using a milling machine. Fifty grams of green tea powder was macerated in 500 mL of boiling water for 15 minutes. The extract was centrifuged at 3000 × g for 7 minutes and the supernatants were pooled and then lyophilized.

3.3. Determination of Total Polyphenol Content of Green Tea Extracts

The amount of polyphenol was measured by a photometric Folin-Ciocalteu assay using the standard gallic acid calibration curve. The method was based on the reduction of phosphotungstic acid in alkaline solution to phosphotungstic blue (28). Briefly, one mL of GTAE was mixed with one mL of three-fold-diluted Folin-Ciocalteu phenol reagent. Two milliliters of 35% sodium carbonate solution was added to the mixture, which was then shaken thoroughly and diluted to 6 mL by adding 2 mL of water. The mixture was incubated for 30 minutes and blue color formed was measured at 700 nm using a spectrophotometer. A calibration curve of gallic acid was prepared and the results were expressed as mg gallic acid equivalents per gram of dried weight of the tea.

3.4. Cell Culture

PC12 cells were obtained from Pasteur Institute (Tehran, Iran). Cells were maintained at 37ºC in a humidified atmosphere (90%) containing 5% CO2. Cells were grown in RPMI 1640 medium supplemented with 10% (v/v) heat-inactivated fetal bovine serum, 100 U/mL penicillin and 100 µg/mL streptomycin.

3.5. Cell Viability

The viability of cultured cells was determined by assaying the reduction of 3-(4,5-dimethyl thiazol-2-yl)-2,5-diphenyl tetrazolium bromide (MTT) to formazan (29). The study was performed in a 96-well microtiter plate of PC12 cells at a density of 6000 cell/well. After pretreatment with GTAE (7.8 - 62.5 µg/mL) for 24 hours, PC12 cells were exposed to ACR at a final concentration of 10 mmol/L and incubated for 24 hours. Then, cells were treated with MTT solution (final concentration of 0.5 mg/mL in a well) for three hours at 37ºC. The formazan crystals were solublized with dimethyl sulfoxide (DMSO) and the absorbance was measured at 545 nm (630 nm as a reference) in an ELISA reader (Start Fax-2100, UK).

3.6. Experimental Animals

Male Wistar rats weighting 200-250 grams were housed in colony rooms with 12/12 h light/dark cycle at 21 ± 2ºC and had free access to food and water. All animal experiments were performed in accordance with Mashhad University of Medical Sciences, Ethical committee Acts.

3.8. The Behavioral Index (Gait Scores) Examination

After 11 days, the gait scores were examined according to the methods described by LoPachin et al. (30). Rats were placed in a clear plexiglass box and were observed for three minutes. Following observation, a gait score was assigned from 1 to 4, where 1 = normal (unaffected gait); 2 = a slightly affected gait (foot splay, slight hindlimb weakness and spread); 3 = a moderately affected gait (foot splay, moderate hindlimb weakness, moderate limb spread during ambulation); and 4 = a severely affected gait (foot splay, severe hindlimb weakness, dragging hindlimb, inability to rear).

3.9. Statistical Analysis

Results were expressed as mean ± SEM. Statistical analyses were performed with ANOVA followed by Tukey-Kramer test to compare the differences between means. Differences were considered statistically significant when P < 0.05.

4. Results

4.1. Total Polyphenol Content of Green Tea

The total phenolic content was 59.8 mg/g of dried weight.

4.2. Effect of ACR in PC12 Cells

PC12 cells were treated with different concentrations of ACR for 24 hours. Cell viability was measured using MTT test. Treatment of the cells with ACR decreased viability in a dose-dependent manner, as shown in Figure 1. The IC50 (50% inhibitory concentration) value for treatment of PC12 cells with ACR for 24 hours was 10 mmol/L.

4.3. Effect of GTAE on ACR-Induced Cytotoxicity in PC12 Cells

After treating PC12 cells with different concentrations of GTAE (7.8 - 62.5 µg/mL) for 24 hours, the final concentration of 10 mmol/L of ACR was added. After 24 hours of exposure, ACR-induced toxicity was measured using MTT test. The results showed an increase in the cell viability of pretreated cells with GTAE (7.8 - 62.5 µg/mL) compared with ACR group (P < 0.01 and P < 0.001, respectively) which is shown in Figure 2. GTAE alone did not show any cytotoxicity.

4.4. Effect of ACR on the Body Weight in Rats and Protective effect of GTAE

Body weight changes of rats during the treatment are shown in Figure 3. A statistical significant decrease in body weight was observed after 11 days of ACR exposure. Treatment with GTAE decreased body weight after 11 days treatment.

4.5. Effect of ACR on the Behavioral Index (gait Scores) in Rats and Protective Effect of GTAE

A single administration of ACR (50 mg/kg, IP) for 11 days produced severe gait abnormalities in rats (P < 0.001) (Figure 4). Although coadministration of GTAE (6.25, 12.5, 25 and 50 mg/kg) and ACR decreased gait abnormalities, the effect was only significant in a group which received GTAE at a dose of 12.5 mg/kg plus ACR (P < 0.001).

Cell Viability of PC12 Cells After Exposure to Different Concentrations of ACR for 24 Hours
Data Are Expressed as Mean ± SEM of Six Separate Experiments, *** P < 0.001 vs. Control Cells.
Effect of GTAE on ACR-Induced Cytotoxicity in PC12 Cells. Cells Were Pretreated With Different Concentrations of GTAE (7.8-62.5 µg/mL) for 24 Hours
Data are Expressed as Mean ± SEM of Six Separate Experiments (n = 6), *** P < 0.001 vs. Untreated Cells, ## P < 0.01 and ### P < 0.001 vs. ACR Treated Cells.
Effects of GTAE on Body Weight Changes of Rats During the Treatment With ACR (50 mg/kg, IP) for 11 Days
Data Are Expressed as the Mean ± SEM, (n = 6), ***P < 0.001 vs. Control, ## P < 0.01 and ### P < 0.001 vs. ACR.
Effects of GTAE on Behavioral Index (Gait Scores) in Rats During the Treatment With ACR (50 mg/kg, IP) for 11 Days
Data Are Expressed as Mean ± SD, (n = 6), *** P < 0.001 vs. Control, ### P < 0.001 vs. ACR Treated Animals.

5. Discussion

In the present study, protective effects of GTAE on ACR induced cytotoxicity in PC12 cells were evaluated as well as ACR induced neurotoxicity in Wistar rats. Our results showed that ACR reduced cell viability in PC12 cells and exposure to GTAE increased the viability of PC12 cells compared with ACR treated cells. Moreover, GTAE pretreated rats showed a greater behavioral index than that of the control group. In the literature, the toxicity of ACR in different in vitro and in vivo models was observed. ACR activated caspase-3 and increased sub-G1 population in SH-SY5Y cells (11, 31). Furthermore, ACR induced apoptosis in PC12 by increasing the bax/bcl2 ratio and activation of caspase 3, which are attributed to the increase of ROS production in PC12 (24). ACR induced apoptosis in neurons and astrocytes in a time and dose-dependent manner and significantly suppressed the proliferation of neural progenitor cells. In addition, apoptotic and necrotic cell death were enhanced in ACR high doses (32).

ACR monomer is a potent neurotoxin and could induce central and peripheral nervous system damages in humans and animals. ACR induces ataxia, skeletal muscle weakness and weight loss in both humans and animal studies (3, 33). Green tea, an infusion from the leaves of C. sinensis Theaceae, is becoming popular as an antioxidant drug (22, 23). The neuroprotective effect of green tea has been documented in different studies. Green tea polyphenols are believed to have the potential as neuropreventive agents for the treatment of neurodegenerative diseases via antioxidant properties (34). Moreover, green tea extract protected ischemia/reperfusion-induced brain cell death by scavenging oxidative damages of macromolecules and inhibited beta-amyloid-induced PC12 cell death (12).

In addition to neuroprotective effects of green tea, several reports showed that green tea could protect other tissues against oxidative damages. It is known that green tea could reduce the oxidation of LDL, which is an important factor in atherosclerosis (35). Additionally, green tea catechins decreased the oxidative stress and hepatic fibrosis (36). In this study, viability of PC12 cells was decreased after 24 hours of exposure to ACR (10 mmol/L). Pretreatment with GTAE (7.8 - 62.5 µg/mL) increased cell viability in a dose-dependent manner. Because of the strong correlation between ROS production and ACR toxicity (13, 37), it is possible that protective effects of GTAE are attributed to the inhibition of ROS generation. Our results showed that treatment of animals with ACR (50 mg/kg, IP) for 11 days decreased body weight and induced severe gait abnormalities (score 4), but treatment of animals with GTAE reduced abnormal gait.

Role of green tea in weight loss has been proved previously. In this study, GTAE 50 mg/kg (the highest dose) decreased mean body weight and mean daily food consumption compared to the control groups, but its effect on mean daily food consumption was not significant (data was not shown). Therefore, it could be suggested that other mechanisms including inhibition of the enzymes catechol-o-methyltransferase, acetyl-CoA carboxylase and fatty acid synthase as well as reducing fat absorption via the gut, may be involved in green tea weight reducing effect. According to our results, ACR caused a significant reduction in body weight after 11 days of treatment. Besides, GTAE led to body weight loss, which could be due to antiobesity effect of green tea (17). Our data also indicated that increasing the dose of GTAE (12.5 mg/kg to 50 mg/kg) decreased green tea neuroprotective effect and the effect of GTAE (12.5 mg/kg) on ACR induced neurotoxicity was more than other selected doses. This may be related in part to body weight loss induced by green tea in higher doses.

As green tea is a source of potent antioxidants, it could be suggested that protective effect of GTAE against ACR toxicity, both in vitro and in vivo experiments, may be related to antioxidant effects of green tea and its constituents.

Acknowledgements

References

  • 1.

    Claus A, Carle R, Schieber A. Acrylamide in cereal products: A review. J Cereal Sci. 2008;47(2):118-33. https://doi.org/10.1016/j.jcs.2007.06.016.

  • 2.

    LoPachin RM. The changing view of acrylamide neurotoxicity. Neurotoxicology. 2004;25(4):617-30. [PubMed ID: 15183015]. https://doi.org/10.1016/j.neuro.2004.01.004.

  • 3.

    LoPachin RM. Acrylamide neurotoxicity: neurological, morhological and molecular endpoints in animal models. Adv Exp Med Biol. 2005;561:21-37. [PubMed ID: 16438286]. https://doi.org/10.1007/0-387-24980-X_2.

  • 4.

    Bull RA, Hansman GS, Clancy LE, Tanaka MM, Rawlinson WD, White PA. Norovirus recombination in ORF1/ORF2 overlap. Emerg Infect Dis. 2005;11(7):1079-85. [PubMed ID: 16022784]. https://doi.org/10.3201/eid1107.041273.

  • 5.

    Tyl RW, Friedman MA. Effects of acrylamide on rodent reproductive performance. Reprod Toxicol. 2003;17(1):1-13. [PubMed ID: 12507653].

  • 6.

    Tareke E, Rydberg P, Karlsson P, Eriksson S, Tornqvist M. Analysis of acrylamide, a carcinogen formed in heated foodstuffs. J Agric Food Chem. 2002;50(17):4998-5006. [PubMed ID: 12166997].

  • 7.

    Deng H, He F, Zhang S, Calleman CJ, Costa LG. Quantitative measurements of vibration threshold in healthy adults and acrylamide workers. Int Arch Occup Environ Health. 1993;65(1):53-6. [PubMed ID: 8394840].

  • 8.

    Sega GA, Alcota RP, Tancongco CP, Brimer PA. Acrylamide binding to the DNA and protamine of spermiogenic stages in the mouse and its relationship to genetic damage. Mutat Res. 1989;216(4):221-30. [PubMed ID: 2761561].

  • 9.

    Working PK, Bentley KS, Hurtt ME, Mohr KL. Comparison of the dominant lethal effects of acrylonitrile and acrylamide in male Fischer 344 rats. Mutagenesis. 1987;2(3):215-20. [PubMed ID: 3325746].

  • 10.

    Friedman MA, Dulak LH, Stedham MA. A lifetime oncogenicity study in rats with acrylamide. Fundam Appl Toxicol. 1995;27(1):95-105. [PubMed ID: 7589934].

  • 11.

    Sumizawa T, Igisu H. Apoptosis induced by acrylamide in SH-SY5Y cells. Arch Toxicol. 2007;81(4):279-82. [PubMed ID: 16932918]. https://doi.org/10.1007/s00204-006-0145-6.

  • 12.

    Lee SY, Lee JW, Lee H, Yoo HS, Yun YP, Oh KW, et al. Inhibitory effect of green tea extract on beta-amyloid-induced PC12 cell death by inhibition of the activation of NF-kappaB and ERK/p38 MAP kinase pathway through antioxidant mechanisms. Brain Res Mol Brain Res. 2005;140(1-2):45-54. [PubMed ID: 16153742]. https://doi.org/10.1016/j.molbrainres.2005.07.009.

  • 13.

    Zhu YJ, Zeng T, Zhu YB, Yu SF, Wang QS, Zhang LP, et al. Effects of acrylamide on the nervous tissue antioxidant system and sciatic nerve electrophysiology in the rat. Neurochem Res. 2008;33(11):2310-7. [PubMed ID: 18470611]. https://doi.org/10.1007/s11064-008-9730-9.

  • 14.

    Suzuki Y, Miyoshi N, Isemura M. Health-promoting effects of green tea. Proc Jpn Acad Ser B Phys Biol Sci. 2012;88(3):88-101. [PubMed ID: 22450537].

  • 15.

    Kuroda Y, Hara Y. Antimutagenic and anticarcinogenic activity of tea polyphenols. Mutat Res. 1999;436(1):69-97. [PubMed ID: 9878691].

  • 16.

    Fujiki H, Suganuma M. Green tea and cancer prevention. Proc Jpn Acad Ser B Phys Biol Sci. 2002;78(9):263-70. https://doi.org/10.2183/pjab.78.263.

  • 17.

    Thavanesan N. The putative effects of green tea on body fat: an evaluation of the evidence and a review of the potential mechanisms. Br J Nutr. 2011;106(9):1297-309. [PubMed ID: 21810286]. https://doi.org/10.1017/S0007114511003849.

  • 18.

    Rains TM, Agarwal S, Maki KC. Antiobesity effects of green tea catechins: a mechanistic review. J Nutr Biochem. 2011;22(1):1-7. [PubMed ID: 21115335]. https://doi.org/10.1016/j.jnutbio.2010.06.006.

  • 19.

    Kuriyama S, Shimazu T, Ohmori K, Kikuchi N, Nakaya N, Nishino Y, et al. Green tea consumption and mortality due to cardiovascular disease, cancer, and all causes in Japan: the Ohsaki study. JAMA. 2006;296(10):1255-65. [PubMed ID: 16968850]. https://doi.org/10.1001/jama.296.10.1255.

  • 20.

    Sabu MC, Smitha K, Kuttan R. Anti-diabetic activity of green tea polyphenols and their role in reducing oxidative stress in experimental diabetes. J Ethnopharmacol. 2002;83(1-2):109-16. [PubMed ID: 12413715].

  • 21.

    Sugiyama K, He P, Wada S, Tamaki F, Saeki S. Green tea suppresses D-galactosamine-induced liver injury in rats. Biosci Biotechnol Biochem. 1998;62(3):609-11. [PubMed ID: 9571796].

  • 22.

    Jun X, Deji S, Ye L, Rui Z. Comparison of in vitro antioxidant activities and bioactive components of green tea extracts by different extraction methods. Int J Pharm. 2011;408(1-2):97-101. [PubMed ID: 21310224]. https://doi.org/10.1016/j.ijpharm.2011.02.002.

  • 23.

    Sung H, Nah J, Chun S, Park H, Yang SE, Min WK. In vivo antioxidant effect of green tea. Eur J Clin Nutr. 2000;54(7):527-9. [PubMed ID: 10918460].

  • 24.

    Mehri S, Abnous K, Mousavi SH, Shariaty VM, Hosseinzadeh H. Neuroprotective effect of crocin on acrylamide-induced cytotoxicity in PC12 cells. Cell Mol Neurobiol. 2012;32(2):227-35. [PubMed ID: 21901509]. https://doi.org/10.1007/s10571-011-9752-8.

  • 25.

    Hosseinzadeh H, Tabeshpur J, Mehri S. Effect of Saffron extract on Acrylamide- induced toxicity: In vitro and in vivo assessment. Chin J Integ Med. 2015;In Press.

  • 26.

    Mehri S, Karami HV, Hassani FV, Hosseinzadeh H. Chrysin reduced acrylamide-induced neurotoxicity in both in vitro and in vivo assessments. Iran Biomed J. 2014;18(2):101-6. [PubMed ID: 24518551].

  • 27.

    Motamedshariaty VS, Amel Farzad S, Nassiri-Asl M, Hosseinzadeh H. Effects of rutin on acrylamide-induced neurotoxicity. Daru. 2014;22(1):27. [PubMed ID: 24524427]. https://doi.org/10.1186/2008-2231-22-27.

  • 28.

    Jun X. Extraction of polyphenolic antioxidants from green tea by ultrahigh pressure technique. 2012. Available from: http://www.paper.edu.cn/en_releasepaper/downPaper/201205-254.html.

  • 29.

    Mousavi SH, Tavakkol-Afshari J, Brook A, Jafari-Anarkooli I. Role of caspases and Bax protein in saffron-induced apoptosis in MCF-7 cells. Food Chem Toxicol. 2009;47(8):1909-13. [PubMed ID: 19457443]. https://doi.org/10.1016/j.fct.2009.05.017.

  • 30.

    LoPachin RM, Ross JF, Reid ML, Das S, Mansukhani S, Lehning EJ. Neurological evaluation of toxic axonopathies in rats: acrylamide and 2,5-hexanedione. Neurotoxicology. 2002;23(1):95-110. [PubMed ID: 12164553].

  • 31.

    Okuno T, Matsuoka M, Sumizawa T, Igisu H. Involvement of the extracellular signal-regulated protein kinase pathway in phosphorylation of p53 protein and exerting cytotoxicity in human neuroblastoma cells (SH-SY5Y) exposed to acrylamide. Arch Toxicol. 2006;80(3):146-53. [PubMed ID: 16180010]. https://doi.org/10.1007/s00204-005-0022-8.

  • 32.

    Park HR, Kim MS, Kim SJ, Park M, Kong KH, Kim HS, et al. Acrylamide induces cell death in neural progenitor cells and impairs hippocampal neurogenesis. Toxicol Lett. 2010;193(1):86-93. [PubMed ID: 20035847]. https://doi.org/10.1016/j.toxlet.2009.12.015.

  • 33.

    Shipp A, Lawrence G, Gentry R, McDonald T, Bartow H, Bounds J, et al. Acrylamide: review of toxicity data and dose-response analyses for cancer and noncancer effects. Crit Rev Toxicol. 2006;36(6-7):481-608. [PubMed ID: 16973444]. https://doi.org/10.1080/10408440600851377.

  • 34.

    Nie G, Cao Y, Zhao B. Protective effects of green tea polyphenols and their major component, (-)-epigallocatechin-3-gallate (EGCG), on 6-hydroxydopamine-induced apoptosis in PC12 cells. Redox Rep. 2002;7(3):171-7. [PubMed ID: 12189048]. https://doi.org/10.1179/135100002125000424.

  • 35.

    Luo M, Kannar K, Wahlqvist ML, O'Brien RC. Inhibition of LDL oxidation by green tea extract. Lancet. 1997;349(9048):360-1. [PubMed ID: 9024405].

  • 36.

    Kobayashi H, Tanaka Y, Asagiri K, Asakawa T, Tanikawa K, Kage M, et al. The antioxidant effect of green tea catechin ameliorates experimental liver injury. Phytomedicine. 2010;17(3-4):197-202. [PubMed ID: 20092986]. https://doi.org/10.1016/j.phymed.2009.12.006.

  • 37.

    Yousef MI, El-Demerdash FM. Acrylamide-induced oxidative stress and biochemical perturbations in rats. Toxicology. 2006;219(1-3):133-41. [PubMed ID: 16343728]. https://doi.org/10.1016/j.tox.2005.11.008.