Anti-leishmanial effect of nanosilver solutions on Leishmania tropica promastigotes by in-vitro assay

authors:

avatar Ahmad Khosravi 1 , avatar Iraj Sharifi 2 , * , avatar Mohammad Barati 3 , avatar Mahdi Zarean 4 , avatar Marayam Hakimi-Parizi 5

MPH student &DVM, Dermatology & Leishmaniasis Research Center, Kerman University of Medical Sciences, Kerman, Iran
Proffesor of Parasitology, Dermatology & Leishmaniasis Research Center, Kerman University of Medical Sciences, Kerman, Iran.
PhD student of Parasitology, School of Hygiene, Tehran University of Medical Sciences, Tehran, Iran.
Instructor of Parasitology, Dermatology & Leishmaniasis Research Center, Kerman University of Medical Sciences, Kerman, Iran
Instructor of Medical Entomology, Dermatology & Leishmaniasis Research Center, Kerman University of Medical Sciences, Kerman, Iran.

how to cite: Khosravi A, Sharifi I, Barati M, Zarean M, Hakimi-Parizi M. Anti-leishmanial effect of nanosilver solutions on Leishmania tropica promastigotes by in-vitro assay. Zahedan J Res Med Sci. 2011;13(7):e93813.

Abstract

Background: At present, nanosilver particles are used against a broad spectrum of microbial agents. The objective of this study was to evaluate the anti-leishmanial effect of nanosilver solutions on L. tropica promastigotes as compared with the trivalent antimony compound by colorimetric assay.
Materials and Method: Promastigots of L. tropica were cultured in RPMI-1640 consisting of 10% fetal calf serum (FCS) and antibiotics at 24±10C. The effect of various concentrations of nanosilver solutions was evaluated in comparison with tartar emetic. Optical density as the result of tetrazolium salts (MTT) reduction by the promastigotes into a formozan product and it was calculated by Elisa reader and IC50 (50% inhibitory concentration), subsequently.
Results: Both, nanosilver particles and tartar emetic inhibited the growth of L. tropica promastigotes. The IC50 for nanosilver solutions was significantly higher (14.9 µg/mL) than the corresponding value (5.3 µg/mL) for the control drug (p<0.001).
Conclusion: Since nanosilver particles showed an effective anti-leishmanial activity, further works are required to evaluate the effect in animal model or human volunteers.

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