Kowsar Jentashapir J Cell Mol Biol 2717-2724 12 1 2021 3 10 10.5812/jjcmb.112986 Mina Zamani Department of Biology, Faculty of Science, Shahid Chamran University of Ahvaz, Ahvaz, Iran Hamid Galehdari Department of Biology, Faculty of Science, Shahid Chamran University of Ahvaz, Ahvaz, Iran Babak Bakhshinejad Department of Genetics, Faculty of Biological Sciences, Tarbiat Modares University, Tehran, Iran Mohammad-Reza Hajjari Department of Biology, Faculty of Science, Shahid Chamran University of Ahvaz, Ahvaz, Iran Ali-Mohammad Foroughmand Department of Biology, Faculty of Science, Shahid Chamran University of Ahvaz, Ahvaz, Iran Research Article 2021 1 28 2021 1 14 2021 3 10 2021 03 31 Background : The association between the human chromosomal 8q24 region and cancer development remains dim. The proto-oncogene MYCis known as the most prominent target of this chromosomal region. However, numerous cancer-associated genetic alterations in the region extend beyond the MYClocus. Accordingly, it is likely that the MYConcogene is not the only target of these carcinogenesis-related alterations. Objectives : In the present study, the expression of MYCand the correlation between MYCand two non-coding RNAs, namely PVT1(circular and linear forms) and CASC11, which are residents of the 8q24 region in the MYCneighborhood, were investigated in chronic myeloid leukemia (CML). Methods : Real-time polymerase chain reaction (PCR) was used to assess BCR-ABLtranscripts and categorize positive and negative (normal) samples for CML. Afterward, real-time PCR was exploited to evaluate the expression of different genes, including MYC, linear PVT1, circular PVT( CircPVT1), CASC11, and ACTBin CML and normal samples. Results : We found that the expression of linear PVT1is significantly increased in CML compared with normal samples. However, CircPVT1, CASC11, and MYCdid not show significantly altered expression between CML and normal groups. The experimental and in silico analyses of the correlation coefficients of gene expressions suggested changes in the correlations between the gene expressions in CML compared with normal samples. We also assessed the miR-trapping potential of PVT1and CASC11and the possible effects of these interactions on signaling pathways. Our findings indicated that these lncRNAs could have a possible regulatory link with critical pathways associated with leukemogenesis. Conclusions : Our results indicate that non-coding genes surrounding MYCwithin the 8q24 region might have regulatory roles in CML carcinogenesis. https://sites.kowsarpub.com/jjcmb/articles/112986.html https://jjcmb.kowsarpub.com/cdn/dl/933434bc-8192-11eb-8158-eb7af53b13d5 CML 8q24 <i>MYC</i> Non-coding RNAs