Briefland Jundishapur J Microbiol 2008-4161 14 10 2021 11 17 10.5812/jjm.117488 Clemente Cruz-Cruz Hospital Juarez of Mexico, Mexico City, Mexico, Higher School of Medicine, National Polytechnic Institute, Mexico City, Mexico Emilio Mariano Durán-Manuel Hospital Juarez of Mexico, Mexico City, Mexico, Higher School of Medicine, National Polytechnic Institute, Mexico City, Mexico Laura Delgado-Balbuena Hospital Juarez of Mexico, Mexico City, Mexico Juan Carlos Bravata-Alcántara Hospital Juarez of Mexico, Mexico City, Mexico Laura Margarita Márquez-Valdelamar Institute of Biology, National Autonomous University of Mexico, Mexico City, Mexico Gabriela Ibáñez-Cervantes Hospital Juarez of Mexico, Mexico City, Mexico Iliana Alejandra Cortés-Ortíz Hospital Juarez of Mexico, Mexico City, Mexico Mónica Alethia Cureño-Díaz Hospital Juarez of Mexico, Mexico City, Mexico Josué Delgado-Balbuena National Institute of Agricultural and Livestock Forestry Research CENID, Jalisco, Mexico Gustavo Esteban Lugo-Zamudio Hospital Juarez of Mexico, Mexico City, Mexico Juan Manuel Bello-López Hospital Juarez of Mexico, Mexico City, Mexico Research Article 2021 10 23 2021 7 13 2021 11 17 2021 10 31 Background : Escherichia coliin the vagina includes several virulence factors in its genome mobile genetic elements and can facilitate colonization, mainly in immunosuppressed patients. Objectives : This work aimed to demonstrate that E. colistrains of vaginal origin isolated from dysplastic patients possess virulence and resistance genes Methods : This study included one hundred and five E. colistrains isolated from women with cervical dysplasia and vaginal infection. The strains were characterized by antimicrobial susceptibility. The Clermont algorithm performed the phylogenetic assignment. The structure of class 1 integrons was performed by identifying integrase ( int1), the variable region, and qacEΔ1-sul1genes. The variable region was amplified, sequenced, and analyzed. Enterobacterial repetitive intergenic consensus (ERIC) PCR and virus typing typed strains with identical genetic arrangements by detecting virulence genes related to cytotoxicity, adherence, and iron uptake. Results : Escherichia colistrains showed great resistance to β-lactams and quinolones, and phylogenetic assignment showed that the group A/C was highly predominant. Sixteen integrons were identified, with monogenic arrays represented by aadA1, dfrB4 dfrA7, dfr2D, and dfrA17cassettes. The prevalence of the biogenic arrays aadA1/dfrA1and aadA5/dfrA17was lower than that of bla _OXA-1 /aadA1. Concerning virulence genes, fimH, traT, and iutAwere the most predominant. Conclusions : The high incidence of virulence and resistance factors in commensal and virulent strains of E. colirevealed potential tools in the pathogenesis of vaginal infection. https://sites.kowsarpub.com/jjm/articles/117488.html https://jjm.kowsarpub.com/cdn/dl/274d9212-5c6a-11ec-817d-c3f8ac2c1fed Antimicrobial Resistance Class 1 Integron, Dysplastic Patients Vaginal Infection Virulence Factor