Briefland Jundishapur J Nat Pharm Prod 2228-7876 16 4 2021 8 16 10.5812/jjnpp.109399 Mehdi Evazalipour Department of Pharmaceutical Biotechnology, School of Pharmacy, Guilan University of Medical Sciences, Rasht, Iran Pouya Safarzadeh Kozani Department of Medical Biotechnology, Faculty of Paramedicine, Guilan University of Medical Sciences, Rasht, Iran, Student Research Committee, Medical Biotechnology Research Center, School of Nursing, Midwifery, and Paramedicine, Guilan University of Medical Sciences, Rasht, Iran Pooria Safarzadeh Kozani Department of Medical Biotechnology, Faculty of Medical Sciences, Tarbiat Modares University, Tehran, Iran Sahar Shabani Department of Pharmacology and Toxicology, School of Pharmacy, Guilan University of Medical Sciences, Rasht, Iran Bahar Rezaei Soufi Student Research Committee, School of Pharmacy, Guilan University of Medical Sciences, Rasht, Iran Ehsan Zamani Department of Pharmacology and Toxicology, School of Pharmacy, Guilan University of Medical Sciences, Rasht, Iran Research Article 2021 2 16 2020 9 13 2021 8 16 2021 11 30 Background : Stress-induced cellular senescence is a perpetual state of cell cycle arrest occurring in proliferating cells in response to stressful conditions. It is believed that oxidative stress plays a unique role in this process. As a reactive chemical compound that can induce oxidative stress, acrylamide is widely applied in several fields. Carvacrol is a liquid phenolic monoterpenoid found in essential oils of some plants and is known for its antioxidant and anticarcinogenic properties. Objectives : The current study aimed to evaluate the effects of carvacrol on oxidative stress and cellular senescence induced by acrylamide in the NIH 3T3 cell line. Methods : NIH 3T3 embryonic fibroblast cells were exposed to different concentrations of acrylamide, carvacrol, and H ₂O ₂in a cell culture medium. The level of β-galactosidase (SA-β-gal) activity, as a marker of cellular senescence, was measured using staining and quantitative assays. Furthermore, to measure oxidative stress parameters, the content of glutathione and lipid peroxidation were determined. Results : Acrylamide could induce premature senescence evident by the elevated lipid peroxidation and SA-β-gal activity and declined cell viability and glutathione. Moreover, carvacrol showed beneficial effects on both acrylamide- and H ₂O ₂-induced cellular senescence by significantly reversing or subsiding the effect of oxidative stress and mediating its consequences. Conclusions : It can be concluded that carvacrol has protective effects against oxidative cellular senescence induced by acrylamide in the NIH 3T3 cell line. https://sites.kowsarpub.com/jjnpp/articles/109399.html https://jjnpp.kowsarpub.com/cdn/dl/aef19338-522b-11ec-a7f6-6bf30f74d8d0 Acrylamide Carvacrol Oxidative Stress Senescence Fibroblasts