Frequency of Shiga Toxin-Producing Genes of Escherichia Coli Isolated from Diarrheic Stools of Iranian Children by PCR

Lida  Nasrollahi; Sedigheh Shams; Mohammad-Taghi Haghi-Ashtiani; Reza Shahsiah; Maryam Monajemzadeh; Behnoosh Tahbaz-Lahafi; Alireza Alaie-Alamooti

IJ Pediatrics

The Scientific Journal of Growth & Development Research Center

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Frequency of Shiga Toxin-Producing Genes of Escherichia Coli Isolated from Diarrheic Stools of Iranian Children by PCR

Author(s):

Lida Nasrollahi^1,*,

Sedigheh Shams²,

Mohammad-Taghi Haghi-Ashtiani³,

Reza Shahsiah⁴,

Maryam Monajemzadeh⁵,

Behnoosh Tahbaz-Lahafi⁶,

Alireza Alaie-Alamooti⁷

IJ Pediatrics:Vol. 23, issue 6; 637-642

Published online:Nov 08, 2013

Article type:Research Article

Received:Jan 20, 2013

Accepted:Aug 14, 2013

How to Cite:Lida NasrollahiSedigheh ShamsMohammad-Taghi Haghi-AshtianiReza ShahsiahMaryam MonajemzadehBehnoosh Tahbaz-LahafiAlireza Alaie-Alamootiet al.Frequency of Shiga Toxin-Producing Genes of Escherichia Coli Isolated from Diarrheic Stools of Iranian Children by PCR.Iran J Pediatr.23(6):637-642.

Abstract

Objective: Shiga toxin-producing E. coli (STEC) is a pathogenic E. coli that may cause hemolytic uremic syndrome (HUS) after diarrheal disease through Shiga toxins. Management of the patients with STEC infection is different from that of other diarrheal diseases due to increase in frequency of HUS after antibiotic administration. Few studies were conducted in Iran and epidemiology of STEC remains obscure; this necessitates examination of stools especially in young children for this bacterium. Methods: We determined the frequency of STEC in 947 E. coli strains isolated from diarrheal stools of children less than 14 years in Tehran with conventional culture methods and multiplex-PCR via determining the STX1 and STX2 genes, between October 2008 and September 2009. We also evaluated the association between stool exam findings and presence of STEC. Findings: Twenty seven (2.8%) of E. coli isolates were positive for STX1 or STX2 genes, most of which occurred in spring (P

Keywords

Shiga Toxin

E. coli

Hemolytic Uremic Syndrome

Polymerase Chain Reaction

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