Germ Tube Formation in Candida Albicans:

Isibor J O; Eghubare A E; Omoregie R

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Germ Tube Formation in Candida Albicans:

Author(s):

Isibor J O^1,*,

Eghubare A E²,

Omoregie R²

1Department of Medical Laboratory Sciences, Faculty of Pathological Sciences, College of Medicine, Ambrose Alli University, P.M.B. 14, Ekpoma, Nigeria.

2School of Medical Laboratory Sciences, University of Benin Teaching Hospital, Benin City, Nigeria

Shiraz E-Medical Journal:Vol. 6, issue 1 and 2; 31837

Published online:Apr 04, 2005

Article type:Research Article

Received:Oct 08, 2014

Accepted:Dec 05, 2014

How to Cite:Isibor J OEghubare A EOmoregie RGerm Tube Formation in Candida Albicans:.Shiraz E-Med J.6(1 and 2):31837.

Abstract

Using the method of Elmer et al (11), the germ tube test was performed for 10 previously confirmed germ-tube positive Candida albicans isolates, using sera obtained from human, rabbit, sheep, cow and goat blood. The possibility of germ tube formation within 30 minutes has been further confirmed in this study. Comparism of mean time of germ tube formation in the sera used showed a significant difference for cow and goat sera (P<0.05), with human serum inducing germ tubes at a faster rate. There was no significant differences (P>0.05) for both rabbit and sheep sera. Using Student t-test analysis, there was significant difference (p<0.05) when different atmospheric conditions of incubation were compared, with a faster rate of formation of germ tubes in 10% CO2. The preferential use of rabbit or sheep sera for demonstrating germ tube formation is suggested, to forestall possible transmission of infectious pathogens through human blood

Keywords

Candida albicans

Germ-Tube Formation

Human and Animal Sera

Incubation Atmosphere

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