In Vitro Antimicrobial Activity of Iris pseudacorus and Urtica dioica

authors:

avatar Maryam Ramtin 1 , * , avatar Alireza Massiha 2 , avatar Mohammad Reza Majid Khoshkholgh - Pahlavian 3 , avatar Khosro Issazadeh 3 , avatar Mehdi Assmar 3 , avatar Saied Zarrabi 4

Graduate of Microbiology and Member of Young Researchers Club of Lahijan, Islamic Azad University of Lahijan Branch, Lahijan, Iran
Department of Microbiology , Member of Young Researchers Club of Lahijan, Lahijan Branch , Islamic Azad University , Lahijan , Iran
Department of Microbiology , Faculty of Science, Lahijan Branch, Islamic Azad University, Lahijan, Iran
Department of Biochemistry , F aculty of Science, Lahijan Branch, Islamic Azad University, Lahijan, Iran

how to cite: Ramtin M , Massiha A, Majid Khoshkholgh - Pahlavian M R, Issazadeh K, Assmar M, et al. In Vitro Antimicrobial Activity of Iris pseudacorus and Urtica dioica. Zahedan J Res Med Sci. 2014;16(3): 35-39.

Abstract

Background: In this study, the effects of antibacterial activity of Urtica dioica and Iris pseudacorus essential oils, native plant northern of Iran, were investigated for some selected bacteria.
Materials and Methods: The influence of essential oils was tested by the using of disk diffusion and micro-broth dilution methods against standard strains of the picked out bacteria. Gas Chromatography/Mass Spectroscopy (GC/MS) analysis, bioactivity determination, Minimum inhibitory concentrations (MIC) and minimum bactericidal concentration (MBC) of essential oils were utilized for this goal.
Results: This study showed that, Inhibition zone diameter varied from 11 to 19 mm and 9 to 17 mm for Urtica dioica and Iris pseudacorus respectively. In contrast, this figure fluctuated from 19 to 28 mm and 7 to 17 mm for gentamicin and ampicillin separately. By the application of micro-broth dilution technique, MICs for 1% essential oils were 1.8-7.5 μg/ml and 3.75-15 μg/ml for, Urtica dioica and Iris pseudacorus against gram-positive and gram-negative bacteria individually. Furthermore, the MBCs of herbal essences were 1.8-15 μg/ml for, Urtica dioica and 15-30 μg/ml for Iris.
Conclusion: The application of essential oils for the bio-control of diseases, as a novel emerging alternative to antimicrobial treatments, lead to safer and more environmental management for infective diseases.

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