Prevalence of Campylobacter Jejuni and Coli in Sheep Carcasses by Using Cultural and PCR Methods

authors:

avatar Reza Shahrokhabadi 1 , * , avatar Ebrahim Rahimi 2 , avatar Hassan Mommtaz 3 , avatar Rahele Poursahebi 4

Young Researchers Club, Shahr-e-Kord Branch, Islamic Azad University, Shahr-e-Kord, Iran
Department of Food Hygiene, Faculty of Veterinary Medicine, Shahr-e-Kord Branch, Islamic Azad University, Shahr-e-Kord, Iran
Department of Microbiology, Faculty of Veterinary Medicine, Shahr-e-Kord Branch, Islamic Azad University, Shahr-e-Kord, Iran
Department of Food Hygiene, Faculty of Veterinary Medicine, Bahonar University, Kerman, Iran

how to cite: Shahrokhabadi R, Rahimi E, Mommtaz H, Poursahebi R. Prevalence of Campylobacter Jejuni and Coli in Sheep Carcasses by Using Cultural and PCR Methods. Zahedan J Res Med Sci. 2013;15(12):e4331.

Abstract

Background: Campylobacter species are common bacterial pathogens causing gastroenteritis in humans worldwide.
Materials and Methods: A total of 148 randomly sheep carcasses were sampled by surface section of neck meat taken immediately after slaughter analyzed using microbiological examinations.
Results: Campylobacter spp. was isolated from 10.13% meat cultures samples examined. Among these 80% sample were C. jejuni and 20% sample were C. coli. Using PCR assays, the number of positive campylobacters increased to 11.48%. Of these positive samples, 82.35% were C. jejuni and 17.65% were C. coli. Significantly higher prevalence rates of Campylobacter spp. (p<0.05) were found in the meat samples taken in summer (47.05%).
Conclusion: The PCR is a reliable and sensitive method which can be used as a diagnostic technique for the detection of campylobacter in lamb samples.

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© 2013, Zahedan Journal of Research in Medical Sciences. This is an open-access article distributed under the terms of the Creative Commons Attribution-NonCommercial 4.0 International License (http://creativecommons.org/licenses/by-nc/4.0/) which permits copy and redistribute the material just in noncommercial usages, provided the original work is properly cited.