Materials and Methods : This research is a quasi-experimental study with repeated measurements in which 14 active female and 13 male subjects with age range 22-24 participated voluntarily. Then, blood was taken from brachial vein of the subjects in three stages before and after GXT (Graded exercise test) and 3 hours after that and SYBER Green PCR Master mix reagent Kit and Real time-PCR were used to measure Cu/Zn SOD mRNA and spectrophotometer was used to measure MDA level.
Results: MDA levels increased significantly in men during the recovery stage and after the exercise (p1=0.012 and p2 =0.014), but it did not increase significantly in active women. Also, MDA difference between the two genders was not reported significant in any of the exercise stages. Cu/Zn SOD gene expression did not increase significantly in either sex.
Conclusion: The risk of injury from free radicals is more probable in active men than active women and vigorous physical activity does not significantly increase the Cu/Zn SOD gene expression.
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