Abstract
Biochemical identification of Mycobacteria after primary culture is a laborious and timeconsuming
procedure. One of the suitable ways for overwhelming meaning this problems is
using high performance liquid chromatography (HPLC) of their Mycolic acids and one of
the most important materials for this process is phenacyl derivatives of high molecular
weight Mycolic acids which is used as standard in this method. This standard is synthesized
by an American company and is not available every time. So, this study is done for
extraction, derivatization and purification of high molecular weight Mycolic acids from
Mycobacterium bovis BCG as standard in the identification of Mycobacteria by means of
HPLC.
Used bacterium in this study was M.bovis BCG and its high molecular weight Mycolic
acids were derivatized by para bromophenacyl bromide and 18- Crown 6 and extracted by
Lich. RP 18 column and methanol/chloroform gradient.
Chromatographs of company synthesized standard and prepared one’s were compared
and retention time of related peaks were recorded.
Complete similarities between chromatographs of commercially produced standard and
provided standard suggest their uniqueness. So, this prepared standard can be used in
identification of Mycobacteria.
procedure. One of the suitable ways for overwhelming meaning this problems is
using high performance liquid chromatography (HPLC) of their Mycolic acids and one of
the most important materials for this process is phenacyl derivatives of high molecular
weight Mycolic acids which is used as standard in this method. This standard is synthesized
by an American company and is not available every time. So, this study is done for
extraction, derivatization and purification of high molecular weight Mycolic acids from
Mycobacterium bovis BCG as standard in the identification of Mycobacteria by means of
HPLC.
Used bacterium in this study was M.bovis BCG and its high molecular weight Mycolic
acids were derivatized by para bromophenacyl bromide and 18- Crown 6 and extracted by
Lich. RP 18 column and methanol/chloroform gradient.
Chromatographs of company synthesized standard and prepared one’s were compared
and retention time of related peaks were recorded.
Complete similarities between chromatographs of commercially produced standard and
provided standard suggest their uniqueness. So, this prepared standard can be used in
identification of Mycobacteria.
Keywords
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References
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Copyright
© 2003, Author(s). This open-access article is available under the Creative Commons Attribution 4.0 (CC BY 4.0) International License (https://creativecommons.org/licenses/by/4.0/), which allows for unrestricted use, distribution, and reproduction in any medium, provided that the original work is properly cited.