Derivatization, Extraction and Purification of High Molecular Weight Mycolic Acid of Mycobacterium bovis BCG by HPLC

Taghi Naserpour Farivar; a Hawaii; gh Naderi; h Tamizifar

Zahedan Journal of Research in Medical Sciences

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Derivatization, Extraction and Purification of High Molecular Weight Mycolic Acid of Mycobacterium bovis BCG by HPLC

Author(s):

Taghi Naserpour Farivar^1,*,

a Hawaii²,

gh Naderi³,

h Tamizifar²

1Microbiology dept, faculty of medicine, Zahedan University of Medical Sciences and health services, Zahedan, Iran.

2Microbiology dept, faculty of medicine, Isfahan University of Medical Sciences and health services, Isfahan, Iran.

3Cardiac research center. Isfahan University of Medical Sciences and health Services, Isfahan, Iran.

Zahedan Journal of Research in Medical Sciences:Vol. 5, issue 1; e95129

Published online:Mar 25, 2003

Article type:Research Article

Received:Mar 02, 2003

Accepted:Mar 17, 2003

How to Cite:Taghi Naserpour Farivara Hawaiigh Naderih TamizifarDerivatization, Extraction and Purification of High Molecular Weight Mycolic Acid of Mycobacterium bovis BCG by HPLC.Zahedan J Res Med Sci.5(1):e95129.

Abstract

Biochemical identification of Mycobacteria after primary culture is a laborious and timeconsuming
procedure. One of the suitable ways for overwhelming meaning this problems is
using high performance liquid chromatography (HPLC) of their Mycolic acids and one of
the most important materials for this process is phenacyl derivatives of high molecular
weight Mycolic acids which is used as standard in this method. This standard is synthesized
by an American company and is not available every time. So, this study is done for
extraction, derivatization and purification of high molecular weight Mycolic acids from
Mycobacterium bovis BCG as standard in the identification of Mycobacteria by means of
HPLC.
Used bacterium in this study was M.bovis BCG and its high molecular weight Mycolic
acids were derivatized by para bromophenacyl bromide and 18- Crown 6 and extracted by
Lich. RP 18 column and methanol/chloroform gradient.
Chromatographs of company synthesized standard and prepared one’s were compared
and retention time of related peaks were recorded.
Complete similarities between chromatographs of commercially produced standard and
provided standard suggest their uniqueness. So, this prepared standard can be used in
identification of Mycobacteria.

Keywords

HPLC

Mycobacterium

Mycolic acids

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References

1.
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