Fulltext
Full text is available in pdf
e-ISSN 2383-1960
p-ISSN 2383-1979
Image Credit:Ann Mil Health Sci Res
Outline
Application of L-Arginine as nitric oxide inducer and Indomethacin as prostaglandin inhibitor in Balb/c mice infected with Leishmania major MRHO/IR/75/ER
Authors
1Researcher, AJA University of Medical Science, Department of Biochemistry, Tehran, Iran, Andorra
2Resercher, Department of Parasitology, Pasteur Institute of Iran, Tehran, Iran, Andorra
*Corresponding Author: Corresponding author: Fatemeh Ghasemi, Researcher, AJA University of Medical Science, Department of Biochemistry, Tehran, Iran, Andorra Email: elhamgha_2007@yahoo.com
Published online:Jan 05, 2013
Article type:Research Article
Received:Sep 25, 2012
Accepted:Dec 05, 2012
How to Cite:Ghasemi F, Nahrevanian H. Application of L-Arginine as nitric oxide inducer and Indomethacin as prostaglandin inhibitor in Balb/c mice infected with Leishmania major MRHO/IR/75/ER. Ann Mil Health Sci Res. 2013;11(1):e66701. doi:
Abstract
Background: Leishmaniasis is one of the health problems in. This study is designed to inhibit PG production by Indomethacin and induce NO by L- Arginine precursor in L. major infected Balb/c mice.
Materials and methods: This was an experimental study. Animals, Male inbred Balb/c mice were used in this study. The total number of animals used in this experiment was 48 Balb/c mice, divided into 6 groups (n =8 mice/group) including Group 1 (naive), Group 2 (L. major + 0.4% Ethanol), Group 3 (L. major + Indomethacin), Group 4 (L. major + DW), Group 5 (L. major + L- Arginine) and Group 6 (L. major + Indomethacin + LArginine). In addition to serum, liver and spleen suspensions were investigated for NO induction by using Griess microassay. The data was analyzed by Analysis of Variances (ANOVA) and Student’s t-test using Graph Pad Prism Software.
Results: The results indicated that production of NO was inhibited in infected Balb/c mice by L. major as compared with naive animals (Group 1). INDO as inhibitor PG (Group 2) showed their ability to elevate RNI levels in infect animals. INDO showed anti-leishmanial activity, as these compounds reduced the lesion sizes (P<0.001). INDO as inhibitor PG (Group 3) showed ability to decrease PG levels in infect animals.
Conclusion: Our data may indicate a possible role for L-Arg and INDO as novel drug targets for the treatment of cutaneous leishmaniasis in mouse model.
Highlights
References
- 1.References are available in pdf.
Copyright
© 2013, Annals of Military and Health Sciences Research. This open-access article is available under the Creative Commons Attribution-NonCommercial 4.0 (CC BY-NC 4.0) International License (https://creativecommons.org/licenses/by-nc/4.0/), which allows for the copying and redistribution of the material only for noncommercial purposes, provided that the original work is properly cited.
Similar Articles
19
Jul
2011
Effect of daily injection of morphine on leishmaniose infection in mice
Ali Gorgeen Karaji,
Mahin Akbari,
Somaye Shabani,
Gholam Reza Bahrami
Gorgeen Karaji A, Akbari M, Shabani S, Bahrami GR. Effect of daily injection of morphine on leishmaniose infection in mice. J Kermanshah Univ Med Sci. 2011;15(2):e79374. doi:
7
Oct
2018
The In Vitro Effect of Hydroalcoholic Extract of Artemisia absinthium on the Growth of Leishmania major (MRHO/IR/75/ER) in Peritoneal Macrophages from BALB/c Mice
Ali Rahiminejad,
Khosrow Hazrati Tappeh,
Shahram Seyyedi,
Peyman Mikaili
Rahiminejad A, Hazrati Tappeh K, Seyyedi S, Mikaili P. The In Vitro Effect of Hydroalcoholic Extract of Artemisia absinthium on the Growth of Leishmania major (MRHO/IR/75/ER) in Peritoneal Macrophages from BALB/c Mice. Jundishapur J Microbiol. 2018;11(11):e77302. doi: https://doi.org/10.5812/jjm.77302
31
Jul
2018
Apoptotic Effect of Morphine, Imiquimod and Nalmefene on Promastigote, Infected and Uninfected Macrophages with Amastigote of Leishmania Major by Flow Cytometry
Parisa Ebrahimisadr,
Fatemeh Ghaffarifar,
John Horton,
Abdolhosein Dalimi,
Zohreh Sharifi
Ebrahimisadr P, Ghaffarifar F, Horton J, Dalimi A, Sharifi Z. Apoptotic Effect of Morphine, Imiquimod and Nalmefene on Promastigote, Infected and Uninfected Macrophages with Amastigote of Leishmania Major by Flow Cytometry. Iran J Pharm Res. 2018;17(3):e124787. doi: https://doi.org/10.22037/ijpr.2018.2262
31
Oct
2019
In-vitro and In-vivo Antileishmanial Activity of a Compound Derived of Platinum, Oxaliplatin, against Leishmania Major
Ezatollah Ghasemi,
Fatemeh Ghaffarifar,
Abdolhossein Dalimi,
Javid Sadraei
Ghasemi E, Ghaffarifar F, Dalimi A, Sadraei J. In-vitro and In-vivo Antileishmanial Activity of a Compound Derived of Platinum, Oxaliplatin, against Leishmania Major. Iran J Pharm Res. 2019;18(4):e126267. doi: https://doi.org/10.22037/ijpr.2019.15364.13046
1
Jul
2014
Peganum harmala Aqueous and Ethanol Extracts Effects on Lesions Caused by Leishmania major (MRHO/IR/75/ER) in BALB/c Mice
Fariba Khoshzaban,
Fatemeh Ghaffarifar,
Hamid Reza Jamshidi Koohsari
Khoshzaban F, Ghaffarifar F, Jamshidi Koohsari HR. Peganum harmala Aqueous and Ethanol Extracts Effects on Lesions Caused by Leishmania major (MRHO/IR/75/ER) in BALB/c Mice. Jundishapur J Microbiol. 2014;7(7):e10992. doi: https://doi.org/10.5812/jjm.10992
Cited by
- Scopus by DOI: 0
Last Update: 1 week ago
- Scopus by Title: 0
Last Update: 1 week ago
- Scopus by Title (Ref): 0
Last Update: 1 week ago
- CrossRef: 0
Last Update: 6 hours ago
- OpenAlex: 0
Last Update: 10 hours ago
- OpenCitations: 0
Last Update: 5 days ago
Comments
Number of Comments:0