Patients and methods:
Totally, 304 HIV-infected individuals referring to Center for Counseling of Behavioral Changes in Shiraz entered our study. Their demographic data and risk factors for HIV (injection addiction, unprotected sexual contact, and blood transfusion) were recorded. Blood specimens were provided and tested for HIV antibodies using an enzyme-linked immunosorbent assay (ELISA) at Fars Blood Transfusion Organization were carried out. Specimens reactive upon enzyme-linked immunosorbent assay were confirmed by western blot assay.