Archives of Clinical Infectious Diseases
Staphylococcus aureus is a major pathogen in hospital setting and in the community and causes a wide range of diseases. MRSA infection has recently become a serious problem in anti-microbial chemotherapy. The aim of the study was to detect and analyze the antibiotic diversity and isolation of methicillin resistance gene (mecA) of S. aureus isolated from Tehran hospitals as a rapid and reliable method.
We studied 585 isolates of staphylococcus spp. recovered from patients at 3 clinical centers in Tehran from October 2005 to October 2006. Antibiotic susceptibility test of isolates was achieved with 13 antibiotics by disc diffusion. The MIC of methicillin was also performed by broth micro dilution assay. PCR was used for detection of mecA gene.
Totally, 321 (54.7%) isolates were identified as S. aureus. 66, 65, 88, 88, 100, 41, 38, 41, 0, 40, 93, 20 and 64% of S. aureus isolates were resistant to kanamycin, cephotaxim, methicillin, oxacillin, ampicillin, erythromycin, clindamycin, sulphamethoxazole-trimethoprime, vancomycin, chloramphenicol, ciprofloxacin, gentamicin and tetracycline, respectively. All MRSA and 63% of intermediate isolates carried mecA gene.
In contrary to other studies in Iran, the prevalence of methicillin resistance is rising up in Tehran and most of MRSA isolates were resistance to 5 antibiotics at least. Vancomycin, chloramphenicol, gentamicin and clindamycin are the most effective antibiotics. All MRSA isolates had mecA gene with different expression. Detection of mecA gene is a rapid and reliable method for identification of MRSA isolates.
© 2009, Author(s). This open-access article is available under the Creative Commons Attribution 4.0 (CC BY 4.0) International License (https://creativecommons.org/licenses/by/4.0/), which allows for unrestricted use, distribution, and reproduction in any medium, provided that the original work is properly cited.
Fatehi Fazli SA, Fatahi-Bafghii M, Vakili M, Sadeh M. Methicillin-Resistant Staphylococcus aureus Isolated from Central Region of Iran: Antibiotic Resistance and SCCmecA Types. Jundishapur J Microbiol. 2024;17(12):e156747. doi: https://doi.org/10.5812/jjm-156747
Rahimi F, Bouzari M, Katouli M, Pourshafie MR. Antibiotic Resistance Pattern of Methicillin Resistant and Methicillin Sensitive Staphylococcus aureus Isolates in Tehran, Iran. Jundishapur J Microbiol. 2013;6(2):144-149. doi: https://doi.org/10.5812/jjm.4896
Nikbakht M, Ahangarzadeh Rezaee M, Hasani A, Nahaei MR, Sadeghi J, et al. Molecular Characterization and Antimicrobial Susceptibility Patterns of Methicillin-Resistant Staphylococcus aureus Isolates in Tabriz, Northwest of Iran. Arch Pediatr Infect Dis. 2017;5(4):e61620. doi: https://doi.org/10.5812/pedinfect.61620
Zarkesh Esfahani FS, Ghandehari F, Nasr Esfahani B, Beheshti-Maal K. Molecular Characterization and Phage Typing of Methicillin-resistant Staphylococcus aureus Isolated from Clinical Samples in Isfahan, Iran. Jundishapur J Microbiol. 2023;16(10):e139277. doi: https://doi.org/10.5812/jjm-139277
Hesari Y, Sohrabi N, Abiri R, Babaei S, Amiri Z. Staphylococcal Cassette Chromosome mec Typing of Meticillin-Resistant Staphylococcus aureus in Kermanshah Province, West of Iran. Jundishapur J Microbiol. 2020;13(3):e98852. doi: https://doi.org/10.5812/jjm.98852
Last Update: 3 weeks ago
Last Update: 3 weeks ago
Last Update: 3 weeks ago
Last Update: 6 days ago
Ordering Reprints
Articles are published under the Creative Commons license stated on each article. No permission or royalty fee is required for uses permitted by that license. CCC handles optional bulk and customized reprint orders. Any quotation covers production and delivery services only, not copyright permission. > Request Reprints from CCC
Author(s):
