Full Text
Full text is available in PDF
Official Journal of Research Center for Gastroenterology and Liver Diseases
e-ISSN 1735-3408
p-ISSN 1735-143X
Evaluation of Diagnostic Value of Elisa Method (EIA) and PCR in Diagnosis of Hepatitis C Virus In Hemodialysis Patients
Authors
1Dept. of Clinical Biochemistry, Urmia School of Medicine, mhansari1@hotmail.com, Azarbayejan-e-Gharbi, IR.Iran
2Genetic Dept., Urmia School of Medicine, Azarbayejan-e-Gharbi, IR.Iran
*Corresponding Author: Dept. of Clinical Biochemistry, Urmia School of Medicine, mhansari1@hotmail.com, Azarbayejan-e-Gharbi, IR.Iran. Tel: +98 441 2223675. Email: mhansari1@hotmail.com
Published online:Jun 30, 2006
Article type:Research Article
Received:Jan 01, 2006
Accepted:Jan 30, 2006
How to Cite:Khadem Ansari M, Omrani M. Evaluation of Diagnostic Value of Elisa Method (EIA) and PCR in Diagnosis of Hepatitis C Virus In Hemodialysis Patients. Hepat Mon. 2006;6(1):. doi:
Abstract
Background and Aims:Since hepatitis C Virus has contaminated approximately 170 millions people over the world. Applying serological screening methods using EIA has led to decline the risk of transmission. However, in chronic renal disease and hemodialysis patients, the EIA method is not sufficient for diagnosis of HCV. Therefore, PCR method is suggested for rapid diagnosis of the disease in this group of patients.
Methods: Of 50 collected blood specimens from hemodialysis patients serum separated and stored at -20º C. Then they were examined by the methods of EIA and PCR.
Results: Using EIA method, 19 cases were positive (38%) and 31 cases were found negative (62%). In PCR method, 12 cases were found positive (24%) and 38 (76%) were negative. In this method, 12 cases were found real positive and 38 cases were found real negative. While, using EIA method, 5 cases were real positive and 24 cases were real negative and 14 cases were found false positive and 7 cases false negative.
Conclusions: Considering the obtained results and their comparison, it is observed that the PCR method is accredited as a specific and reliable method suitable for public screening and that the PCR method is recommended as an exact and final diagnosis method of these patients.
Highlights
Copyright
© 2006, Author(s). This open-access article is available under the Creative Commons Attribution 4.0 (CC BY 4.0) International License (https://creativecommons.org/licenses/by/4.0/), which allows for unrestricted use, distribution, and reproduction in any medium, provided that the original work is properly cited.
Similar Articles
30
May
2013
Hepatitis C virus (HCV) Infection Rate among Seronegative Hemodialysis Patients Screened by Two Methods; HCV Core Antigen and Polymerase Chain Reaction
Maryam Moini,
Mazyar Ziyaeyan,
Shapoor Aghaei,
Mohammad Mahdi Sagheb,
Seyed Alireza Taghavi,
Mahsa Moeini
,et al.Moini M, Ziyaeyan M, Aghaei S, Sagheb MM, Taghavi SA, et al. Hepatitis C virus (HCV) Infection Rate among Seronegative Hemodialysis Patients Screened by Two Methods; HCV Core Antigen and Polymerase Chain Reaction. Hepat Mon. 2013;13(6):e9147. doi: https://doi.org/10.5812/hepatmon.9147
6
Feb
2017
The Prevalence of HCV Infection in Hemodialysis Population and Compared ELISA and PCR Methods for Detecting of HCV Infection
Atieh Makhlough,
Mohammadreza Mahdavi,
Iradj Maleki,
Lotfollah Davoodi,
Tahoora Mousavi,
Seyed-Hosein Hasani-Mansoor
,et al.Makhlough A, Mahdavi M, Maleki I, Davoodi L, Mousavi T, et al. The Prevalence of HCV Infection in Hemodialysis Population and Compared ELISA and PCR Methods for Detecting of HCV Infection. Nephro-Urol Mon. 2017;9(3):e45144. doi: https://doi.org/10.5812/numonthly.45144
26
Dec
2021
Prevalence of Hepatitis C Infection and its Genotypes in Suspected Hemodialysis Patients, Southwest of Iran
Marzieh Jamalidoust,
Maryam Eskandari,
Mazyar Ziyaeyan
Jamalidoust M, Eskandari M, Ziyaeyan M. Prevalence of Hepatitis C Infection and its Genotypes in Suspected Hemodialysis Patients, Southwest of Iran. Jundishapur J Microbiol. 2021;14(11):e118591. doi: https://doi.org/10.5812/jjm.118591
28
Jul
2023
Prevalence of Hepatitis C Virus and Its Occult Infection in Hemodialysis Patients
Mohsen Nakhaie,
Ehsan Taheri,
Javad Charostad,
Nasir Arefinia,
Davood Kalantar-Neyestanaki,
Mohammad Rezaei Zadeh Rukerd
,et al.Nakhaie M, Taheri E, Charostad J, Arefinia N, Kalantar-Neyestanaki D, et al. Prevalence of Hepatitis C Virus and Its Occult Infection in Hemodialysis Patients. Jundishapur J Microbiol. 2023;16(5):e136504. doi: https://doi.org/10.5812/jjm-136504
30
Jul
2022
Comparing RT-qPCR and Hepatitis C Virus Antigen Detection Assay for Detecting Active Infection in Blood Donors in Fars Province, Iran
Leila Kasraian,
Ali Farhadi,
Gholamreza Rafiei Dehbidi,
Mehdi Mirzakhani,
Sedigheh Sharifzadeh,
Sepide Namdari
,et al.Kasraian L, Farhadi A, Rafiei Dehbidi G, Mirzakhani M, Sharifzadeh S, et al. Comparing RT-qPCR and Hepatitis C Virus Antigen Detection Assay for Detecting Active Infection in Blood Donors in Fars Province, Iran. Hepat Mon. 2022;22(1):e123438. doi: https://doi.org/10.5812/hepatmon-123438
Cited by
- Scopus by DOI: 0
Last Update: 3 weeks ago
- Scopus by Title: 0
Last Update: 3 weeks ago
- Scopus by Title (Ref): 0
Last Update: 3 weeks ago
- CrossRef: 0
Last Update: 5 hours ago
- OpenAlex: 0
Last Update: 2 days ago
- OpenCitations: 0
Last Update: 4 days ago
Comments
Number of Comments:0