A study of genotypes, mutants and nucleotide sequence of hepatitis B virus in Pakistan

Khalid Mumtaz; Saeed Hamid; Shahid Ahmed; Tariq Moatter; Shamim Mushtaq; Anis Khan; Masashi Mizokami; Wasim Jafri

Hepatitis Monthly

Official Journal of Research Center for Gastroenterology and Liver Diseases

Current Issue Accepted Manuscripts All Issues In Press Articles Search

Journal Information Boards and committees Indexing and Listing Sources Journal Metrics Open Peer Review (OPR)Publication Ethics and Malpractice Statement Reviewer and AE Registration Form Support Contact Us

APC

Authors Guide Submit Manuscript

A study of genotypes, mutants and nucleotide sequence of hepatitis B virus in Pakistan

Author(s):

Khalid Mumtaz^1,*,

Saeed Hamid²,

Shahid Ahmed²,

Tariq Moatter²,

Shamim Mushtaq²,

Anis Khan³,

Masashi Mizokami³,

Wasim Jafri²

1Departments of Medicine and Pathology, Aga Khan University, khalid.mumtaz@aku.edu, Pakistan

2Departments of Medicine and Pathology, Aga Khan University, Pakistan

3Department of Clinical Molecular Informative Medicine, Japan

Hepatitis Monthly:Vol. 11, issue 1; 14-18

Published online:Jan 31, 2011

Article type:Research Article

Received:Jul 27, 2010

Accepted:Sep 07, 2010

How to Cite:Mumtaz K, Hamid S, Ahmed S, Moatter T, Mushtaq S, et al. A study of genotypes, mutants and nucleotide sequence of hepatitis B virus in Pakistan.Hepat Mon.2011;11(1):14-18.

Abstract

Background: Hepatitis B virus (HBV) genotypes and mutations are gaining importance in determining the clinical course of chronic liver disease.
Objectives: To determine and compare the distribution of HBV genotypes and genomic variations in Pakistan to other parts of the world.
Patients and Methods: We conducted a prospective study at Aga Khan University Hospital from December 2006 to December 2008. HBV genotype was determined in 257 HBV DNA-positive patients. Patients were divided into two groups according to HBeAg positivity. Mutations in the pre-core and core promoter regions of HBV were determined in HBeAg-negative patients by line probe INNOLIPA assay.
Results: The mean±SD age of patients was 28±5 years; there were 201 (78%) men. HBeAg was positive in 219 (85%) patients and negative in 38 (15%). HBeAg-positive patients were younger than HBeAg-negative patients (95% vs 21% in ≤30 years, p<0.001). HBV genotype D found in 247 (96.2 %) patients followed by a combined infection with HBV genotype B+D in 9 (3.3%) and 1 (0.5%) with genotype A. The mutations identified in 38 HBeAg-negative patients were T1762/A1764 in 21 (55.2%), PC mutant in 7 (18.4%), T1762/A1764/PC mutant in 2 (5%) and T1762/A1764/PC wild mutation in 1 (2%); no mutation identified in 7 (18.4%). Phylogenetic analysis did not show any significant differences between HBV genotype D isolated from Pakistan and those isolated from other parts of the world.
Conclusions: HBV genotype D is predominant in Pakistan, irrespective of HBeAg status. PC and BCP mutations were found in significant numbers of patients infected with genotype D. The HBV genotype D isolates from Pakistan are identical to the sequences isolated from other parts of the world.

Implication for Health policy/practice/research/medical education:
This study magnifies is an interesting article which describes the distribution of different genotypes and mutations of Hepatitis B virus among a normal population in Pakistan. Epidemiologists, basic sciences specialists like geneticians benefit from the results of this article.
Please cite this paper as:
Mumtaz K, Hamid S, Ahmed S, Moatter T, Mushtaq S, Khan A, et al. A study of genotypes, mutants and nucleotide sequence of hepatitis B virus in Pakistan. Hepat Mon. 2011;11(1):14-18.

Keywords

Full Text

Full text is available in PDF

Import into EndNote Import into BibTex

Share on

Comments

Number of Comments:0

Cited by

Scopus by DOI: 0
Last Update: 4 days ago
Scopus by Title: 0
Last Update: 4 days ago
Scopus by Title (Ref): 26
Last Update: 4 days ago
CrossRef: 0
Last Update: 5 days ago

Metrics

Purchasing Reprints

Copyright Clearance Center (CCC) handles bulk orders for article reprints for Brieflands. To place an order for reprints, please click here ( https://www.copyright.com/landing/reprintsinquiryform/ ). Clicking this link will bring you to a CCC request form where you can provide the details of your order. Once complete, please click the ‘Submit Request’ button and CCC’s Reprints Services team will generate a quote for your review.

Search Relations

Author(s):

Khalid Mumtaz:[PubMed][Scholar]
Saeed Hamid:[PubMed][Scholar]
Shahid Ahmed:[PubMed][Scholar]
Tariq Moatter:[PubMed][Scholar]
Shamim Mushtaq:[PubMed][Scholar]
Anis Khan:[PubMed][Scholar]
Masashi Mizokami:[PubMed][Scholar]
Wasim Jafri:[PubMed][Scholar]

Official Journal of Research Center for Gastroenterology and Liver Diseases

Outlines

A study of genotypes, mutants and nucleotide sequence of hepatitis B virus in Pakistan

Abstract

Full Text

Last Update: 4 days ago

Last Update: 4 days ago

Last Update: 4 days ago

Last Update: 5 days ago