Cytogenetic methods for detection of oxidative stress and evaluation of antioxidant therapy in hepatitis C infection

How to Cite:Emerit I. Cytogenetic methods for detection of oxidative stress and evaluation of antioxidant therapy in hepatitis C infection. Hepat Mon. 2011;11(6):. doi:

Abstract

The plasma of patients with hepatitis C contains chromosome-damaging substances, the so-called "clastogenic factors" (CFs), as this is the case for other chronic inflammatory diseases and after radiation exposure. These endogenous clastogens, formed as a consequence of increased superoxide production by inflammatory cells, can be detected with cytogenetic methods, as they are used for exogenous clastogens. The long-lived, autosustained DNA-damaging effects of CFs are risk factors for the development of cancer and leukemia. In hepatitis C, the highest clastogenic scores has been observed in patients with hepatocellular carcinoma. In agreement with the link to inflammation, clastogenic score are correlated with necro-inflammatory scores in liver biopsies. Antioxidant therapy with a powerful superoxide scavenger resulted in normalization of clastogenic scores and significant decreases in aminotransferase levels, but did not influence the virus load. Preliminary results of our study on a limited number of patients suggest that pre-treatment with antioxidants may improve the outcome of interferon/ribavirin treatment. A comparison of a three-month treatment with either interferon alone or the antioxidant alone, yielded similar results for reduction of ALT levels, but only complete normalization of clastogenic scores for the antioxidant. Further studies have to be conducted to see whether a combination of an antiviral agent with an appropriate antioxidant would allow to reduce interferon and its side effects.Combination of antioxidants with IFN/RIBA was also reported by other authors with discordant results. The CF-test can be useful in clinical trials for the choice of the appropriate antioxidant.

Implication for health policy/practice/research/medical education:
The fact that DNA damage induced during chronic hepatitis C may be reduced by antioxidants is of importance for prevention of hepatocellular carcinomas. The CF-test is an appropriate and easy tool to follow chromosome mutation in the disease process.
Please cite this paper as:
Emerit I. Cytogenetic methods for detection of oxidative stress and evaluation of antioxidant therapy in hepatitis C infection. Hepat Mon. 2011;11(6):434-39.

Keywords

Hepatitis C virus

Oxidative stress

Cytogenetic

Full Text

Full text is available in PDF

Oxidative Stress and Benefits of Antioxidant Agents in Acute and Chronic Hepatitis

Mohammad Baghianimoghadam,

Hossein Forghani,

Razieh Zolghadr,

Mohammad Ali Morowati Sharifabad

Baghianimoghadam M, Forghani H, Zolghadr R, Morowati Sharifabad MA. Oxidative Stress and Benefits of Antioxidant Agents in Acute and Chronic Hepatitis. Hepat Mon. 2009;9(4):. doi:

31

Mar

2012

Oxidative Stress and Benefits of Antioxidant Agents in Acute and Chronic Hepatitis

Heqiu Zhang,

Xiaoguo song,

Guohua Wang,

Kun chen,

Bingshui Xiu,

Shigan Ling

,et al.

Zhang H, song X, Wang G, chen K, Xiu B, et al. Oxidative Stress and Benefits of Antioxidant Agents in Acute and Chronic Hepatitis. Hepat Mon. 2008;8(1):. doi:

31

Mar

2012

Oxidative Stress and Benefits of Antioxidant Agents in Acute and Chronic Hepatitis

Mukaddes Esrefoglu

Esrefoglu M. Oxidative Stress and Benefits of Antioxidant Agents in Acute and Chronic Hepatitis. Hepat Mon. 2012;12(3):. doi: https://doi.org/10.5812/hepatmon.837

17

Jul

2016

Hepatitis B and its Relationship With Oxidative Stress

Seyed Moayed Alavian,

Alireza Showraki

Alavian SM, Showraki A. Hepatitis B and its Relationship With Oxidative Stress. Hepat Mon. 2016;16(9):e37973. doi: https://doi.org/10.5812/hepatmon.37973

21

Feb

2015

Oxidative Stress Response in Patients Infected by Diverse Hepatitis C Virus Genotypes

Mohammad Hassan Khadem Ansari,

Mir-Davood Omrani,

Fatemeh Kheradmand

Khadem Ansari MH, Omrani M, Kheradmand F. Oxidative Stress Response in Patients Infected by Diverse Hepatitis C Virus Genotypes. Hepat Mon. 2015;15(2):e22069. doi: https://doi.org/10.5812/hepatmon.22069

Import into EndNote Import into BibTex

Share on

Comments

Number of Comments:0

Cited by

Scopus by DOI: 0
Last Update: 1 week ago
Scopus by Title: 2
Last Update: 1 week ago
Scopus by Title (Ref): 2
Last Update: 1 week ago
CrossRef: 0
Last Update: 2 days ago

Metrics

Ordering Reprints

Articles are published under the Creative Commons license stated on each article. No permission or royalty fee is required for uses permitted by that license. CCC handles optional bulk and customized reprint orders. Any quotation covers production and delivery services only, not copyright permission. > Request Reprints from CCC

Search Relations

Author(s):

Ingrid Emerit:[PubMed][Scholar]

Official Journal of Research Center for Gastroenterology and Liver Diseases

Outlines

Cytogenetic methods for detection of oxidative stress and evaluation of antioxidant therapy in hepatitis C infection

Abstract

Full Text

Similar Articles

Oxidative Stress and Benefits of Antioxidant Agents in Acute and Chronic Hepatitis

Oxidative Stress and Benefits of Antioxidant Agents in Acute and Chronic Hepatitis

Oxidative Stress and Benefits of Antioxidant Agents in Acute and Chronic Hepatitis

Hepatitis B and its Relationship With Oxidative Stress

Oxidative Stress Response in Patients Infected by Diverse Hepatitis C Virus Genotypes

Last Update: 1 week ago

Last Update: 1 week ago

Last Update: 1 week ago

Last Update: 2 days ago