The Evaluation of Hepatitis C Virus Core Antigen in Immunized Balb/C Mice

How to Cite:Torbati E, Pakzad P, Mosaffa N, Koochaki A, Kazemi B, et al. The Evaluation of Hepatitis C Virus Core Antigen in Immunized Balb/C Mice.Hepat Mon.2012;12(6):391-397.https://doi.org/10.5812/hepatmon.6141.

Abstract

Background: Hepatitis infection represents one of the important causes of morbidity and mortality in developing countries, however there is not any effective vaccine against hepatitis C which is one of the significant problems in vaccine project.
Objectives: The aim of the present study is to evaluate the role of HCV core protein in inducing IFN-Gamma secretion and TCL activities as a vaccine in Balb/C mice.
Material and Methods: Our previous cloned plasmid (HCV Core gene into pETDuet-1) applied for protein expression in bacteria. The expressed and purified recombinant protein together with Freund’s adjuvant was injected to 15 Balb/c mice. The total IgG and IgG2a of immunized mice sera were evaluated after a week. Two weeks after booster injection, we studied the proliferation and IFNγ secretion of spleens, inguinal and popliteal lymph nodes lymphocytes by ELISA and ELISPOT.
Results: The FSFC (Frequency of spot forming cells) of secreting cells of immunized mice with HCV/Core protein and sera IgG2a were considerably higher than the control groups.
Conclusions: The core protein together with proper adjuvant can be a candidate vaccine against of HCV infection.

Implication for health policy/practice/research/medical education:
This study has implication for further researches and vaccine development and it is also useful for medical researchers and education specially immunologists.
Please cite this paper as:
Torbati E, Bandehpour M, Pakzad P, Mosaffa N, Kazemi B. The Evaluation of Hepatitis C Virus Core Antigen in Immunized Balb/C Mice. Hepat Mon. 2012;12(6): 391-397. DOI: 10.5812/hepatmon.6141

Keywords

Hepacivirus

Core protein

Recombinant Proteins

Fulltext

Full text is available in PDF

Import into EndNote Import into BibTex

Crossmark

Checking

Share on

Comments

Number of Comments:0

Cited by

Scopus by DOI: 3
Last Update: 3 weeks ago
Scopus by Title: 3
Last Update: 3 weeks ago
Scopus by Title (Ref): 3
Last Update: 3 weeks ago
CrossRef: 2
Last Update: 2 days ago

Metrics

Get Permission (article level)

Purchasing Reprints

Copyright Clearance Center (CCC) handles bulk orders for article reprints for Brieflands. To place an order for reprints, please click here ( https://www.copyright.com/landing/reprintsinquiryform/ ). Clicking this link will bring you to a CCC request form where you can provide the details of your order. Once complete, please click the ‘Submit Request’ button and CCC’s Reprints Services team will generate a quote for your review.

Search Relations

Author(s):

Elham Torbati:[PubMed][Scholar]
Parviz Pakzad:[PubMed][Scholar]
Nariman Mosaffa:[PubMed][Scholar]
Ameneh Koochaki:[PubMed][Scholar]
Bahram Kazemi:[PubMed][Scholar]
Mojgan bandepour:[PubMed][Scholar]

Official Journal of Research Center for Gastroenterology and Liver Diseases

Outlines

The Evaluation of Hepatitis C Virus Core Antigen in Immunized Balb/C Mice

Abstract

Fulltext

Crossmark

Checking

Last Update: 3 weeks ago

Last Update: 3 weeks ago

Last Update: 3 weeks ago

Last Update: 2 days ago