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Journal of Kermanshah University of Medical Sciences
Peer-reviewed Medical Quarterly
e-ISSN 2588-2570
p-ISSN 2588-2562
Image Credit:J Kermanshah Univ Med Sci
Outline
The agreement rate of rose Bengal, modified rose Bengal and rapid Wright tests for detection of positive serum sample of Brucellosis
Authors
1Dept. of Immonology, School of Medicine, Kermanshah University of Medical Sciences, Kermanshah, Iran
2Central Labratory, Kermanshah University of Medical Sciences, Kermanshah, Iran
3Dept. of Biostatistics and Epidemiology, School of Medicine, Kermanshah University of Medical Sciences, Kermanshah, Iran
*Corresponding Author: Corresponding author: Ali Gorgeen Karaji, Dept. of Immonology, School of Medicine, Kermanshah University of Medical Sciences, Kermanshah, Iran, Tel: +98 831 4274618, Fax: +98 831 4276423 Email: ali4459@gmail.com
Published online:May 19, 2011
Article type:Research Article
Received:Jan 29, 2010
Accepted:Jun 06, 2010
How to Cite:Gorgeen Karaji A, Abdi F, Rezaei M. The agreement rate of rose Bengal, modified rose Bengal and rapid Wright tests for detection of positive serum sample of Brucellosis. J Kermanshah Univ Med Sci. 2011;15(1):e69718. doi:
Abstract
Background: Rose Bengal is a common test for serologic diagnosis of brucellosis. The aim of this study was to evaluate the agreement rate between modified rose Bengal, rose Bengal and rapid Wright tests for detection of antibody positive serum samples.
Methods: Cross section study was designed. 941 serum sample of patients referred to the central medical laboratory of Kermanshah, were evaluated by rose Bengal, modified rose Bengal and rapid Wright, using convenient sampling method. Positive samples were confirmed and their titers were defined by Wright agglutination test.
Results: The number of positive serum samples with modified rose Bengal was two fold higher than conventional rose Bengal test (P<0.001), which included all positive samples with rose Bengal. On the other hand, although the number of positive diagnosed serum samples with modified rose Bengal were nearly two times more than Wright test (P<0.001), but it did not include all positive samples detected by Wright test. All three tests showed acceptable agreement rate (k≥0.583).
Conclusion: Results of this study confirmed that modified rose Bengal able to detect more positive serum sample than conventional rose Bengal and it would be substituted rose Bengal. However, the rapid Wright test should be used aside with modified rose Bengal, because it could not cover all positive samples detected by rapid Wright test.
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Copyright
© 2011, Journal of Kermanshah University of Medical Sciences. This open-access article is available under the Creative Commons Attribution-NonCommercial 4.0 (CC BY-NC 4.0) International License (https://creativecommons.org/licenses/by-nc/4.0/), which allows for the copying and redistribution of the material only for noncommercial purposes, provided that the original work is properly cited.
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