Journal of Kermanshah University of Medical Sciences
Peer-reviewed Medical Quarterly
e-ISSN 2588-2570
p-ISSN 2588-2562
Outline
Growth inhibition and Induction of apoptosis in K562 Chronic Myeloid Leukaemia cells by the derivatives of dihydropyrano[c]chromenes family
Authors
1Dept. of Biology, Faculty of Natural Science, University of Tabriz, Tabriz, Iran
2Dept. of Organic Chemistry, K. N. Toosi University of Tech, Tehran, Iran
*Corresponding Author: Corresponding author: Majid Mahdavi, Dept. of Biology, Faculty of Natural Science, University of Tabriz, Tabriz, Iran, Tel: +98 411 3343110 Email: [email protected]
Published online:Oct 29, 2013
Article type:Research Article
Received:Apr 08, 2013
Accepted:Aug 03, 2013
How to Cite:Pejman S, Mahdavi M, Balalaei S. Growth inhibition and Induction of apoptosis in K562 Chronic Myeloid Leukaemia cells by the derivatives of dihydropyrano[c]chromenes family. J Kermanshah Univ Med Sci. 2013;17(7):e74458. doi:
Abstract
Background: Recently, the anticancer effect of Chromene derivatives in different cell types has been reported. In this study, we report some derivatives of dihydropyrano[c]chromenes with growth inhibitory and apoptotic activity against K562, Chronic Myeloid Leukaemia (CML).
Methods: The cells were seeded in 96-well plates at 1×105 cells/well and treated with different concentrations of the dihydropyrano[c]chromenes (4-PC, 4-CNC and 4-HC). Growth inhibition and viability determined by trepan blue exclusion test. Apoptosis was also detected by fluorescent microscopy and DNA fragmentation assay.
Results: The 4-PC, 4-CNC and 4-HC inhibited growth and viability of the cells in dose- and time-dependent manner. These compounds were found to be highly active growth inhibitor with IC50 of 240, 180 and 160 μM, respectively. The results of Acridine orange/ethidium bromide (AO/EtBr) double staining also revealed that the compounds induced apoptosis at respective IC50 value after 72 h of treatment.
Conclusion: As the effect of growth inhibition and induction of apoptosis in K562 Chronic Myeloid Leukaemia cells were observed, dihydropyrano[c]chromenes can be proposed as effective agents for more investigation in leukaemia treatment.
References
- 1.The references of this article is available on PDF.
Copyright
© 2013, Journal of Kermanshah University of Medical Sciences. This open-access article is available under the Creative Commons Attribution-NonCommercial 4.0 (CC BY-NC 4.0) International License (https://creativecommons.org/licenses/by-nc/4.0/), which allows for the copying and redistribution of the material only for noncommercial purposes, provided that the original work is properly cited.
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