Acute effect of diazinon on lipid peroxidation level and activities of antioxidant enzymes in rat spleen

Sedigh Ahmadi; Mahvash Jafari; Alireza  Asgari; Maryam Salehi

Journal of Kermanshah University of Medical Sciences

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Acute effect of diazinon on lipid peroxidation level and activities of antioxidant enzymes in rat spleen

Author(s):

Sedigh Ahmadi¹,

Mahvash Jafari^2,*,

Alireza Asgari³,

Maryam Salehi⁴

1Neurosciences Research Center, Baqiyatallah (a.s) University of Medical Sciences, Tehran, Iran

2Chemical Injuries Research Center, Baqiyatallah (a.s) University of Medical Sciences, Tehran, Iran

3Sport Physiology Research Center, Baqiyatallah (a.s) University of Medical Sciences, Tehran, Iran

4Dept. of Biochemistry, School of Medicine, Baqiyatallah (a.s) University of Medical Sciences, Tehran, Iran

Journal of Kermanshah University of Medical Sciences:Vol. 16, issue 1; e78874

Published online:May 29, 2012

Article type:Research Article

Received:Jun 02, 2011

Accepted:Oct 11, 2011

How to Cite:Ahmadi S, Jafari M, Asgari A, Salehi M. Acute effect of diazinon on lipid peroxidation level and activities of antioxidant enzymes in rat spleen. J Kermanshah Univ Med Sci. 2012;16(1):e78874. doi:

Abstract

Background: Diazinon (DZN) is one of the most organophosphates (OPs) insecticides that widely used in agriculture and industry. Some OPs are capable to produce free radicals and induce malfunction in body antioxidant systems. Enzymatic and nonenzymatic antioxidants such as superoxide dismutase (SOD), catalase (CAT) and glutathione S-transferase (GST) and glutathione (GSH) normally counteract damaging effects of free radicals. The aim of this study was to evaluate the effects of DZN on spleen antioxidant system in male rat.

Methods: Wistar male rats were randomly divided into four groups including: control group receiving corn oil as DZN solvent and three experimental groups receiving of different doses DZN (30, 50 and 100 mg/kg) by intraperitoneal injection. 24 hours after injection, animals were anesthetized and spleen tissue removed. After hemogenizing, SOD, CAT, GST and lactate dehydrogenase (LDH) activities as well as GSH and malondialdehyde (MDA) levels were determined by biochemical methods.

Results: At doses level higher than 30 mg/kg DZN, SOD and GST activities were significantly increased, while CAT activity and GSH level were significantly decreased, when compared with the control.

Conclusion: DZN induces the production of free radicals and oxidative stress in a dose- dependent manner. The alteration of antioxidant enzyme activities in spleen may result from increased detoxification capacity. Depletion of GSH can impair the cell’s defense against the toxic actions of free radicals and indicative of oxidative tissue injury.

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1.
The references of this article is available on PDF.

Evaluation of oxidative stress biomarkers of rat liver exposed to acute doses of diazinon

M Hajigholamali,

M Jafari,

R Hajihossaini,

A Asgari,

M Abasnezhad,

M Salehi

17

Aug

2011

The Comparison of the Effect of Diazinon and Paraoxon on Biomarkers of Oxidative Stress in Rat Serum

Maryam Salehi,

Mahvash Jafari,

Masoud Saleh-Moqadam,

Alireza Asgari

28

Feb

2016

Strain-Related Differences on Response of Liver and Kidney Antioxidant Defense System in Two Rat Strains Following Diazinon Exposure

Maryam Salehi,

Mahvash Jafari,

Alireza Asgari,

Mohammad Salimian,

Maryam Abbasnezhad,

Reza Haji Hosseini

,et al.

2

Dec

2021

Protective effect of resveratrol against sub-acute diazinon-induced oxidative stress in rat kidney

amir hosseinrahbari,

habibollah nazem,

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Fereshteh Mehri

24

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2024

Reduction of Oxidative Stress and Testicular Tissue Damage in Wistar Rat Treated with Diazinon by Quercetin

Ghodrat Ebadimanas,

Gholamreza Najafi

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Author(s):

Sedigh Ahmadi:[PubMed][Scholar]
Mahvash Jafari:[PubMed][Scholar]
Alireza Asgari:[PubMed][Scholar]
Maryam Salehi:[PubMed][Scholar]

Peer-reviewed Medical Quarterly

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Acute effect of diazinon on lipid peroxidation level and activities of antioxidant enzymes in rat spleen

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