Journal of Kermanshah University of Medical Sciences
Peer-reviewed Medical Quarterly
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Identification of species of leishmania isolated from patients with cutaneous leishmaniasis in Kermanshah; using RAPD-PCR technique
Author(s):
1Dept.of Parasitology and Mycology,School of Medicine, Kermanshah University of Medical Sciences, Kermanshah, Iran
2Dept.of Microbiology, School of Medicine, Kermanshah University of Medical Sciences, Kermanshah, Iran
3Dept.of Immunology, School of Medicine, Kermanshah University of Medical Sciences, Kermanshah, Iran
*Corresponding Author: Corresponding author: Yazdan Hamzavi, Dept.of Parasitology and Mycology,School of Medicine, Kermanshah University of Medical Sciences, Kermanshah, Iran, Tel: +98 831 4274622 Email: [email protected]
Published online:Dec 19, 2010
Article type:Brief Report
Received:Jan 10, 2010
Accepted:May 18, 2010
How to Cite:Hamzavi Y, Nomanpour B, Gorgin Karaji A. Identification of species of leishmania isolated from patients with cutaneous leishmaniasis in Kermanshah; using RAPD-PCR technique. J Kermanshah Univ Med Sci. 2010;14(3):e79482. doi:
Abstract
Annually many numbers of pationts with Cutaneous Leishmaniasis (CL) have been reported in Kermanshah province- IRAN. The study aimed to identify species of Leishmania isolated from patients with CT in Kermanshah. Seven isolates of Leishmania obtained from patients with CL, without any travelling to other provinces, were cultured in NNN medium. After mass production of leptomonads in RPMI 1640 medium DNA was purified and the species were diagnosed using RAPD-PCR technique. The study of electrophoretic fingerprints of the product of RAPD-PCR in seven isolates showed that Leshmania major was the causative agent of CL patients in Kermanshah province. More studies in this field recommended.
References
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Copyright
© 2010, Journal of Kermanshah University of Medical Sciences. This open-access article is available under the Creative Commons Attribution-NonCommercial 4.0 (CC BY-NC 4.0) International License (https://creativecommons.org/licenses/by-nc/4.0/), which allows for the copying and redistribution of the material only for noncommercial purposes, provided that the original work is properly cited.
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