Hepatoprotective Effects of Combination Hydroalcoholic Extracts of Nigella Sativa and Curcuma Longa on Adriamycin- Induced Oxidative Stress in Rat

Authors

Reza Mohebbati1, Mohammad Reza Khazdair2, 3, Sareh Karimi1, Abbasali Abbasnezhad4,*
1Department of Physiology, School of Medicine, Medical University of Mashhad, Iran
2Pharmaceutical Research Center and Department of Physiology School of Medicine, Mashhad University of Medical Sciences, Mashhad, Iran
3Student Research Committee, Mashhad University of Medical Sciences, Mashhad, Iran
4Department of Basic Sciences, Faculty of Medicine, Gonabad University of Medical Sciences, Gonabad, Iran
*Corresponding Author: Corresponding author: Department of Basic Sciences, Faculty of Medicine, Gonabad University of Medical Sciences, Gonabad, Iran. Email:[email protected]

Journal of Reports in Pharmaceutical Sciences:Vol. 6, issue 2; 105-114
Published online:Jun 10, 2017
Article type:Research Article
Received:Apr 18, 2016
Accepted:Aug 17, 2016
How to Cite:Mohebbati R, Khazdair MR, Karimi S, Abbasnezhad A. Hepatoprotective Effects of Combination Hydroalcoholic Extracts of Nigella Sativa and Curcuma Longa on Adriamycin- Induced Oxidative Stress in Rat. J Rep Pharm Sci. 2017;6(2):e147608. doi:

Abstract

The ample of studies show that Adriamycin caused hepatotoxicity in rat. The present study investigated the effects of Nigella Sativa (N. Sativa) and combination with Curcuma longa (C. longa) extract on Adriamycin-induced hepatotoxicity in rat.
Rats were divided into eight experimental groups: Control (CO), Adriamycin (ADR), Vitamin C (Vit C), Adriamycin with Vitamin C (ADR+VitC), N. Sativa with and without Adriamycin (NS +ADR, NS -ADR), Combination extract of C. longa and N. Sativa with and without Adriamycin (NS+CL+ADR, NS+CL-ADR). Malondialdehyde (MDA) and thiol levels and also the activities of catalase (CAT) in liver tissue were evaluated.
MDA level in the liver tissue in ADR was increased compared to CO group but in NS+ADR group, ADR+VitC and NS –ADR groups decreased compared to ADR group. Thiol levels in ADR and ADR+VitC groups were decreased compared to CO group. Thiol levels in treatment groups were increased compared to ADR group. The activities of CAT in liver tissue of ADR group were lower than CO group, and increased in treatment groups comparison with ADR group.
The results showed that chronic administration of N. sativa hydroalcoholic extract in Adriamycin-induced hepatotoxicity rats could decrease the oxidative stress injuries in liver tissue.

Copyright

© 2017, Author(s). This open-access article is available under the Creative Commons Attribution 4.0 (CC BY 4.0) International License (https://creativecommons.org/licenses/by/4.0/), which allows for unrestricted use, distribution, and reproduction in any medium, provided that the original work is properly cited.

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