Effects of different therapeutical doses of ibuprofen on the adenosine deaminase activity at physiologic and pathologic temperatures

  Introduction: Adenosine deaminase (ADA) is a purine catabolic enzyme that removes amino group of adenosine or 2' deoxyadenosine irreversibly. ADA enhances immune system and also intervenes the inflammation process. In this study, the effect of ibuprofen as an anti- inflammatory drug has been studied on the ADA activity in the physiologic and pathologic temperatures.   Materials and Methods: ADA was assessed in the presence of 3 different doses of ibuprofen at 37oC and 42oC via spectrometry in 265 nm.   Results: Ibuprofen at high and low doses had an activation effect on the ADA activity at 37oC and moderated the sensitivity of enzyme activity to its substrate. It also decreased the anti-inflammatory effect of adenosine via decrease of its concentration and had a positive effect on immune system as well. The activation of the enzyme by Ibuprofen was decreased at 42oC and also Ibuprofen moderated the effect of temperature on dependency of enzyme activity to its substrate. Ibuprofen effects on immune system and anti-inflammatory effects of adenosine decreased at 42oC.   Conclusion: Ibuprofen is a putative activator for adenosine deaminase and administration of this drug could be useful for immune deficiency. Since there is no activator for ADA so far, this drug is important. Ibuprofen decreases anti-inflammatory effect of adenosine. In-vivo studies would be needed .