Koomesh
Journal of Semnan University of Medical Sciences
Outlines
Cytotoxicity effect of gold nanoparticles against a rabbit model of retinoblastoma
Abstract
Introduction: Retinoblastoma is a malignant tumor in children. The goal of retinoblastoma treatment is to maintain vision and minimize side effects. In this study, the toxicity of the gold nanoparticle safety in vitro was investigated on an eye tumor of retinoblastoma under in vivo conditions. Materials and Methods: For the evaluation of the toxicity of gold nanoparticles using MTT test after 48 hours in a CO2 incubator at 37 ° C, Y79 cell line was administered under in vitro conditions at concentrations of 1.69, 1.75, 3.5, 7, 14 and 28 μg/ml of gold nanoparticles. Safe dose in vitro was extracted. The toxicity of gold nanoparticles in the treatment of retinoblastoma tumor under in vivo conditions in a rabbit animal model using histochemistry analysis and hemoxin-eosin staining, as well as measuring the cross-sectional area of the tumor using ultrasonic sequential image analysis and extraction using software Image J was studied. Results: The results showed that with increasing the concentration of gold nanoparticles, the percentage of live cells in comparison with the control group had a significant difference (p 0.05) in the concentration of 1.69 μg/ml. The results indicated that the injection of gold nanoparticles into the rabbit;#39s eye, with retinoblastoma tumor reduced the size of tumor in the rabbit;#39s eye. Conclusion: It is concluded that the toxicity of gold nanoparticles has a therapeutic effect on Y79 cell line in vitro and in the eye of retinoblastoma tumors in vivo
References
- 1.Castillo BV, Kaufman L. Pediatric tumors of the eye and orbit. Pediatr Clin North Am 2003; 50: 149-172. ##.
- 2.Shields CL, Shields JA. Diagnosis and management of retinoblastoma. Cancer Control 2004; 11: 317-324.##.
- 3.Thampi S, Hetts SW, Cooke DL, Stewart PJ, Robbins E, Banerjee A, Dubois SG, Char D, Halbach V, Matthay K. Superselective intra-arterial melphalan therapy for newly diagnosed and refractory retinoblastoma: results from a single institution. Clin Ophthalmol 2013; 7: 981989. ##.
- 4.Anubhav C, Shashi DS. Chemotherapy in retinoblastoma. Int J Therap Rehabilit Res 2016; 5: 55-60.##.
- 5.Kooshafar Z, Salimi M., Javid A . Evaluating antitumor effect of a novel hydrazide derivative in mammalian mice model. Koomesh, 2018; 20 (3): 582- 587.##.
- 6.Sarkar A, Ghosh M, Sil PC. Nanotoxicity: Oxidative stress mediated toxicity of metal and metal oxide nanoparticles. J Nanosci Nanotechnol 2014; 14: 730-743.##.
- 7.Omidi M, Yadegari A, Zali H, Hashemi M, Hasanzadeh H. Cancer cell detection using electrochemical nano biosensor based on graphene/ gold nanoparticle. Koomesh 2016; 18: 211- 219. ##.
- 8.Rothen-Rutishauser B, Mhlfeld C, Blank F, Musso C, Gehr P. Translocation of particles and inflammatory responses after exposure to fine particles and nanoparticles in an epithelial airway model. Part Fibre Toxicol 2007; 4: 9-18.##.
- 9.Keun SO, Ree SK, Jinho L, Dongmin K, Sun HC, Soon HY. Gold/chitosan/pluronic composite nanoparticles for drug delivery. J Appl Polym Sci 2008; 108: 3239-3244. ##.
- 10.Amanda JH, Lei C, William LK, Richard PVD. Detection of a biomarker for Alzheimers disease from synthetic and clinical samples using a nanoscale optical biosensor. J Am Chem Soc 2005; 127: 2264-2271.##.
- 11.Hongwei L, Colleen LN, Jason HH. Biomedical applications of plasma on resonant metal nanoparticles. Nanomedicine 2006; 1: 201-208.##.
- 12.Fayaz AM, Balaji K, Girilal M, Kalaichelvan PT, Venkatesan RM. Synthesis of silver nanoparticles and their incorporation into sodium alginate films for vegetable and fruit preservation. J Agric Food Chem 2009; 57: 6246- 6252.##.
- 13.Service RF. Nanotoxicology: Nanotechnology grows up. Science 2004; 304: 1732-1734.##.
- 14.Ghosh P, Han G, De M, Kim CH, Rotello VM. Gold nanoparticles in delivery applications. Adv Drug Deliv Rev 2008; 60:1307-1315. ##.
- 15.Shukla R, Bansal V, Chaudhary M, Basu A, Bhonde RR, Sastry M. Biocompatibility of gold nanoparticles and their endocytotic fate inside the cellular compartment: A microscopic overview. Langmuir 2005; 8: 10644-10654. ##.
- 16.Yamanaka YJ, Leong KW. Engineering strategies to enhance nanoparticle mediated oral delivery. J Biomater Sci Polym Ed 2008;19:1549-70.##.
- 17.Braydich-Stolle L, Hussain S, Schlager JJ, Hofmann MC. In vitro cytotoxicity of nanoparticles in mammalian germ line stem cells. Toxic Sci 2005; 88: 412-419. ##.
- 18.Wei D, Qian W. Facile synthesis of Ag and Au nanoparticles utilizing chitosan as a mediator agent. Coll Sur B: Biointerfaces 2008; 62: 136-142.##.
- 19.Shokrzadeh M, Zalzar Z, Ebrahimnejad P. Preparation, characterization and in vitro cytotoxicity evaluation of docetaxel nanoparticles against A549 cell line. Koomesh. Spring 2019; 21 (2): 340-346.##.
- 20.Kang SJ, Grossniklaus HE. Rabbit model of retinoblastoma. J Biomed Biotechnol 2011; 394730: 1-6.##.
- 21.Nel A, Xia T, Madler L, Li N. Toxic potential of materials at the nano-level. Science 2006; 311: 622-627.##.
- 22.Song K, Xu P, Meng Y, Geng F, Li J, Li Z, Xing J, Chen J. Smart gold nanoparticles enhance killing effect on cancer cells. Int J Oncol 2013; 42: 597-608.##.
- 23.Chithrani BD, Chan WC. Elucidating the mechanism of cellular uptake and removal of protein-coated gold nanoparticles of different sizes and shapes. Nano Lett 2007; 7: 1542-1550.##.
- 24.Chen H, Dorrigan A, Saad S, Hare DJ, Cortie MB, Valenzuela SM. In vivo study of spherical gold nanoparticles: Inflammatory effects and distribution in mice. PLOS ONE 2013; 8: e58208.##.
- 25.Cho WK, Kang S, Choi H, Rho CR. Topically administered gold nanoparticles inhibit experimental corneal neovascularization in mice. Cornea 2015; 34: 456-459.##.
- 26.Hao K, Yan Z, Shuang Y, Sun J, Tao S, Fu W. Association between interferon gamma 13-CA-repeats polymorphism and metastasis of nasopharyngeal carcinoma in a population of Northern China. Int J Clin Exp Pathol 2015; 8: 7409-7414.##.
- 27.Tkachenko AG, Xie H, Liu Y, Coleman D, Ryan J, Glomm WR, Shipton MK, Franzen S, Feldheim DL. Cellular trajectories of peptide-modifed gold particle complexes: Comparison of nuclear localization signals and peptide transduction domains. Bioconjug Chem 2004; 15: 482490. ##.
- 28.Martnez-Torres AC, GZarate-Trivio D, Lorenzo-Anota HY, vila-vila A, Rodrguez-Abrego C, Rodrguez-Padilla C. Chitosan gold nanoparticles induce cell death in HeLa and MCF-7 cells through reactive oxygen species production. International Journal of Nanomedicine., 2018; 13: 32353250.##.
- 29.Boyles M , Kristl T, Andosch A, Zimmermann M, Tran N, Casals E, Himly M, Puntes V, Huber C, LutzMeindl U, Duschl A. Chitosan functionalisation of gold nanoparticles encourages particle uptake and induces cytotoxicity and proinflammatory conditions in phagocytic cells, as well as enhancing particle interactions with serum components. J Nanobiotechnol 2015; 13: 1-20.##.
- 30.Jong WHD, Borm PJA. Drug delivery and nanoparticles: Applications and hazards. Int J Nanomed 2008; 3: 133-149.##.
- 31.Cho WS, Cho M, Jeong J, Choi M, Cho HY, Han BS, Kim SH, Kim HO, Lim YT, Chung BH, Jeong J. Acute toxicity and pharmacokinetics of 13 nm-sized PEG-coated gold nanoparticles. Toxicol Appl Pharmacol 2009; 236: 1624.##.
- 32.Chen YS, Hung YC, Liau I, Huang GS. Assessment of the in vivo toxicity of gold nanoparticles. Nanoscal Res Lett 2009; 4: 858864.##.
- 33.Komatsu T, Nakamura K, Okuura Y, Konishi K. Optimal method of gold nanoparticle administration in melanomabearing mice. Exp Therap Med 2018; 15: 2994-2999.##.
Copyright
© 2019, Author(s). This open-access article is available under the Creative Commons Attribution 4.0 (CC BY 4.0) International License (https://creativecommons.org/licenses/by/4.0/), which allows for unrestricted use, distribution, and reproduction in any medium, provided that the original work is properly cited.
Similar Articles
Toxicity Effect of Silver Nanoparticles on Mice Liver Primary Cell Culture and HepG2 Cell Line
Faedmaleki F, H Shirazi F, Salarian A, Ahmadi Ashtiani H, Rastegar H. Toxicity Effect of Silver Nanoparticles on Mice Liver Primary Cell Culture and HepG2 Cell Line. Iran J Pharm Res. 2014;13(1):e125411. doi: https://doi.org/10.22037/ijpr.2014.1436
Gold Nanoparticle and Mean Inactivation Dose of Human Intestinal Colon Cancer HT-29 Cells
Arab-Bafrani Z, Saberi A, Tahmasebi Birgani MJ, Shahbazi-Gahrouei D, Abbasian M, et al. Gold Nanoparticle and Mean Inactivation Dose of Human Intestinal Colon Cancer HT-29 Cells. Jundishapur J Nat Pharm Prod. 2015;10(4):e29153. doi: https://doi.org/10.17795/jjnpp-29153
Study of Protective Effects of Gold Nano Particles on the Liver Toxicity Induced by Carbon-Tetrachloride (CCl4) in Male Rats
Shahraki MR, Mirshekari H, Badini F, Arab MR, Shahraki E. Study of Protective Effects of Gold Nano Particles on the Liver Toxicity Induced by Carbon-Tetrachloride (CCl4) in Male Rats. Zahedan J Res Med Sci. 2017;19(7):e9293. doi: https://doi.org/10.5812/zjrms.9293
Target dose enhancement factor alterations related to interaction between the photon beam energy and gold nanoparticles’ size in external radiotherapy: using Monte Carlo method
Khosravi H, Hashemi B, Mahdavi SR, Hejazi P. Target dose enhancement factor alterations related to interaction between the photon beam energy and gold nanoparticles’ size in external radiotherapy: using Monte Carlo method. koomesh. 2015;17(1):e150799. doi:
Oxidative Stress Induction by Gold Nanoparticles in HCT-116 Colon Cancer Cells
Maddah A, Danesh H, Ziamajidi N, Khosravi H, Abbasalipourkabir R. Oxidative Stress Induction by Gold Nanoparticles in HCT-116 Colon Cancer Cells. Compr Health Biomed Stud. 2023;2(1):e145183. doi: https://doi.org/10.5812/chbs-145183
- Scopus by DOI: 0
Last Update: 1 month ago
- Scopus by Title: 0
Last Update: 1 month ago
- Scopus by Title (Ref): 0
Last Update: 1 month ago
- CrossRef: 0
Last Update: 5 days ago
Ordering Reprints
Articles are published under the Creative Commons license stated on each article. No permission or royalty fee is required for uses permitted by that license. CCC handles optional bulk and customized reprint orders. Any quotation covers production and delivery services only, not copyright permission. > Request Reprints from CCC
Author(s):