Epidemiological study of tuberculosis in Damghan city (Iran) during 2003-2007

Sadegh MohammadiEzni; AliAkbar Mansorian; Zeinab Nokandeh

doi:10.5812/koomesh-153786

Koomesh

Journal of Semnan University of Medical Sciences

Home

All Issues In Press Accepted Manuscripts Search

Instructions

Journal Information Editors & Boards Indexing and Listing Sources Journal Metrics Open Peer Review (OPR)Publication Ethics and Publication Malpractice Statement Reviewer and AE Registration Form Support Contact Us

APC

Authors Guide Submit Manuscript

Epidemiological study of tuberculosis in Damghan city (Iran) during 2003-2007

Author(s):

Sadegh MohammadiEzni^1,*,

AliAkbar Mansorian¹,

Zeinab Nokandeh²

1Damghan Health Center, Semnan University of Medical Sciences, Damghan, Iran

2Dept. of Biology, Islamic Azad University of Damghan, Damghan, Iran.

Koomesh:Vol. 9, issue 4; e153786

Published online:Nov 24, 2008

Article type:Research Article

Received:May 09, 2008

Accepted:Nov 24, 2008

How to Cite:MohammadiEzni S, Mansorian A, Nokandeh Z. Epidemiological study of tuberculosis in Damghan city (Iran) during 2003-2007. koomesh. 2008;9(4):e153786. doi:

Abstract

Background: Leptospirosisis a very common zoonosis in the world. Culture is low sensitive with high rate false negative. So, serological assays are best alternative way for its diagnosis. Microscopic agglutination test (MAT) is gold standard but performing it requires a panel of some standard strains and need periodic subculturing of them, and also requires double sera with at least two weeks interval to investigate seroconversion. Furthermore, other serological methods should be investigated. The aim of this study was to evaluate an in-house IgM-ELISA developed by using antigen extracted from endemic isolates. Material and Method: I4 endemic isolates belonged to the serogroups: Icterohaemorrahgia, Pomona, Hardjo, and Gripotyphosa, were inoculated in EMJH to take well grown cultures. Whole antigen was extracted from each culture by Freezing-Thawing method in distilled water. Same amount of extraction of each culture with same OD number in 550nm were mixed together and were used for coating Elisa plates. Antihuman IgM conjugated with alkaline phosphatase were used in this assay. We used a commercial quantitative IgM-ELISA (SERION ELISA classic) for cut off determination. MAT was used for confirmation positive and negative cases. Sera with titer ≥ 1:100 in MAT and positive criteria in commercial quantitative IgM-ELISA were considered as positive cases. Results: 98 positive cases and 54 negative cases were chosen by screening 200 sera of patients suspected to leptospirosis by using MAT and commercial quantitative IgM-ELISA. We also used 30 sera of patients affected by hepatitis B, salmonelosis, and brucellosis as control cases. 88 of 98 positive cases were positive (false negative=10), 1 of 54 negative and all control case were negative (false positive =1) in the test. Sensitivity, specificity, PPV, NPV, and accuracy of the test were evaluated :99.0% , 89.1% , 90.75 , 98.8% , and 94.25 , respectively. Conclusions: ELISA for measuring specific IgM to leptospires antigen(s) could be a good alternative to MAT, which is not a routine diagnostic assay to perform in clinical diagnostic laboratories and only is reliable when there is paired sera. Sensitivity and specificity of the assay is dependent to several factors, especially to the type of antigen coated on plates, quality of assay materials, and also to the time of sampling. Sera of days ≥ 6 of the disease has enough antibodies to measure and a common antigen extracted from several common pathogenic leptospires, especially from endemic isolates, could be more helpful to increase accuracy of the assay

Keywords

Evaluation an in–house IgM-ELISA for the diagnosis of human leptospirosis

Seyed Mehdi Sadat,

Raheb Ghorbani,

Behroz Kavehei,

Hesamedin Askari Majdabadi,

Abdolkarim Shadmehr

Sadat SM, Ghorbani R, Kavehei B, Askari Majdabadi H, Shadmehr A. Evaluation an in–house IgM-ELISA for the diagnosis of human leptospirosis. koomesh. 2008;9(2):e153764. doi:

24

Nov

2008

Evaluation an in–house IgM-ELISA for the diagnosis of human leptospirosis

Hamidreza Honarmand,

Motahereh Nezafat Tabalvandi,

Abtin Heidarzadeh,

Bahram Soltani,

Ebrahim Mirzajani,

Mehdi Asmar

Honarmand H, Nezafat Tabalvandi M, Heidarzadeh A, Soltani B, Mirzajani E, et al. Evaluation an in–house IgM-ELISA for the diagnosis of human leptospirosis. koomesh. 2008;9(4):e153782. doi:

24

Nov

2008

Evaluation an in–house IgM-ELISA for the diagnosis of human leptospirosis

Gholamreza Toogeh,

Maryam Helali,

Shaban Alizade,

Akbar Dorgalaleh

Toogeh G, Helali M, Alizade S, Dorgalaleh A. Evaluation an in–house IgM-ELISA for the diagnosis of human leptospirosis. J Cell Mol Anesth. 2016;1(2):e150211. doi: https://doi.org/10.22037/jcma.v1i2.11417

24

Nov

2008

Evaluation an in–house IgM-ELISA for the diagnosis of human leptospirosis

Hossein Samiei Abianeh,

Shahram Nazarian,

Abbas Hajizadeh,

Emad Kordbache

Samiei Abianeh H, Nazarian S, Hajizadeh A, Kordbache E. Evaluation an in–house IgM-ELISA for the diagnosis of human leptospirosis. koomesh. 2022;24(6):e154141. doi:

24

Nov

2008

Evaluation an in–house IgM-ELISA for the diagnosis of human leptospirosis

Abdollah HajiVandi,

Hosein Ghaedi

HajiVandi A, Ghaedi H. Evaluation an in–house IgM-ELISA for the diagnosis of human leptospirosis. koomesh. 2006;7(1):e152089. doi:

Import into EndNote Import into BibTex

Share on

Comments

Number of Comments:0

Cited by

Scopus by DOI: 0
Last Update: 6 days ago
Scopus by Title: 6
Last Update: 6 days ago
Scopus by Title (Ref): 0
Last Update: 6 days ago
CrossRef: 0
Last Update: 7 hours ago

Metrics

Ordering Reprints

Articles are published under the Creative Commons license stated on each article. No permission or royalty fee is required for uses permitted by that license. CCC handles optional bulk and customized reprint orders. Any quotation covers production and delivery services only, not copyright permission. > Request Reprints from CCC

Search Relations

Author(s):

Sadegh MohammadiEzni:[PubMed][Scholar]
AliAkbar Mansorian:[PubMed][Scholar]
Zeinab Nokandeh:[PubMed][Scholar]

Journal of Semnan University of Medical Sciences

Outlines

Epidemiological study of tuberculosis in Damghan city (Iran) during 2003-2007

Abstract

Similar Articles

Evaluation an in–house IgM-ELISA for the diagnosis of human leptospirosis

Evaluation an in–house IgM-ELISA for the diagnosis of human leptospirosis

Evaluation an in–house IgM-ELISA for the diagnosis of human leptospirosis

Evaluation an in–house IgM-ELISA for the diagnosis of human leptospirosis

Evaluation an in–house IgM-ELISA for the diagnosis of human leptospirosis

Last Update: 6 days ago

Last Update: 6 days ago

Last Update: 6 days ago

Last Update: 7 hours ago