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A Rapid Modified Antibody-Capture Enzyme-Linked Immunosorbent Assay (ELISA) for Detection HSV-1 Primary Infection in Human
Authors
+Corresponding Author: Department of Virology, Tarbiat Modarres University, Tehran, Iran., ravanshad@modares.ac.ir. Tel: +98(21)8801-1001. Fax: +98 (21) 8801-3030. Email: ravanshad@modares.ac.ir
Published online:Apr 01, 2006
Article type:Research Article
How to Cite:Ravanshad M. A Rapid Modified Antibody-Capture Enzyme-Linked Immunosorbent Assay (ELISA) for Detection HSV-1 Primary Infection in Human. Shiraz E-Med J. 2006;7(2):20366. doi:
Abstract
An antibody-capture enzyme-linked immunosorbent assay (ELISA) based on the reaction between anti human IgM and serum IgM and HSV-1 conjugated with horseradish peroxidase (HRP) was developed. The specificity and sensitivity of the developed HSV-1 IgM ELISA were evaluated and compared with a commercially available test kit by using sets of well defined sera of known HSV-1 primary infection. For validation, 526 serum samples have been tested. The HSV-1 IgM ELISA had acceptable sensitivity and specificity, which were comparable with that of the commercial test kit. Both assays showed some cross-reactivity with sera containing anti-HSV-2 antibodies
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Copyright
© 2006, Author(s). This open-access article is available under the Creative Commons Attribution 4.0 (CC BY 4.0) International License (https://creativecommons.org/licenses/by/4.0/), which allows for unrestricted use, distribution, and reproduction in any medium, provided that the original work is properly cited.
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