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Zahedan Journal of Research in Medical Sciences
The Official Journal of Zahedan University of Medical Sciences
e-ISSN 2383-2894
Image Credit:Zahedan J Res Med Sci
Outline
Detection of Enterotoxigenic Staphylococcus aureus in Schizothorax zarudnyi Using PCR Method
Authors
1Student of master of science of genetics, University of Zabol, Zabol, Iran
2D epartment of F ood S afety , Faculty of Veterinary, University of Zabol, Zabol, Iran
*Corresponding Author: Student of master of science of genetics, University of Zabol, Zabol, Iran Email: nargesahani@hotmail.com
Published online:Mar 06, 2013
Article type:Brief Report
Received:Jan 09, 2013
Accepted:Feb 05, 2013
How to Cite:Ahani N, Alipour - Eskandani M. Detection of Enterotoxigenic Staphylococcus aureus in Schizothorax zarudnyi Using PCR Method. Zahedan J Res Med Sci. 2014;16(4):. doi:
Abstract
Background: Enterotoxins of Staphylococcus aureus are the main factors which cause food poisoning.
Matrrials and Methods: Thirty five samples of Schizothorax zarudnyi were cultured by bacteriological methods and S. aureuses were identified. The isolated bacteria were evaluated by PCR for diagnosis of the gene encoding of SEA and SEB. Data were analyzed using Stata software.
Results: PCR is a rapid, sensitive, specific and inexpensive method for detecting Staphylococcal enterotoxins in food.
Conclusion: 37.2% of fishes were contaminated by S. aureus. The PCR results showed that 14.3% of S. aureus isolates possessed the SEA gene, 8.5% had the SEB gene and 5.7% possessed both genes.
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References
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Copyright
© 2014, Author(s). This open-access article is available under the Creative Commons Attribution 4.0 (CC BY 4.0) International License (https://creativecommons.org/licenses/by/4.0/), which allows for unrestricted use, distribution, and reproduction in any medium, provided that the original work is properly cited.
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