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Zahedan Journal of Research in Medical Sciences
The Official Journal of Zahedan University of Medical Sciences
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In Vitro Antimicrobial Activity of Iris pseudacorus and Urtica dioica
Author(s):
Maryam Ramtin1,*, Alireza Massiha2, Mohammad Reza Majid Khoshkholgh - Pahlavian3, Khosro Issazadeh3, Mehdi Assmar3, Saied Zarrabi 41Graduate of Microbiology and Member of Young Researchers Club of Lahijan, Islamic Azad University of Lahijan Branch, Lahijan, Iran
2Department of Microbiology , Member of Young Researchers Club of Lahijan, Lahijan Branch , Islamic Azad University , Lahijan , Iran
3Department of Microbiology , Faculty of Science, Lahijan Branch, Islamic Azad University, Lahijan, Iran
4Department of Biochemistry , F aculty of Science, Lahijan Branch, Islamic Azad University, Lahijan, Iran
*Corresponding Author: Graduate of Microbiology and Member of Young Researchers Club of Lahijan, Islamic Azad University of Lahijan Branch, Lahijan, Iran Email: [email protected]
Published online:Feb 12, 2013
Article type:Research Article
Received:Oct 14, 2012
Accepted:Dec 18, 2012
How to Cite:Ramtin M, Massiha A, Majid Khoshkholgh - Pahlavian MR, Issazadeh K, Assmar M, et al. In Vitro Antimicrobial Activity of Iris pseudacorus and Urtica dioica. Zahedan J Res Med Sci. 2014;16(3):. doi:
Abstract
Background: In this study, the effects of antibacterial activity of Urtica dioica and Iris pseudacorus essential oils, native plant northern of Iran, were investigated for some selected bacteria.
Materials and Methods: The influence of essential oils was tested by the using of disk diffusion and micro-broth dilution methods against standard strains of the picked out bacteria. Gas Chromatography/Mass Spectroscopy (GC/MS) analysis, bioactivity determination, Minimum inhibitory concentrations (MIC) and minimum bactericidal concentration (MBC) of essential oils were utilized for this goal.
Results: This study showed that, Inhibition zone diameter varied from 11 to 19 mm and 9 to 17 mm for Urtica dioica and Iris pseudacorus respectively. In contrast, this figure fluctuated from 19 to 28 mm and 7 to 17 mm for gentamicin and ampicillin separately. By the application of micro-broth dilution technique, MICs for 1% essential oils were 1.8-7.5 μg/ml and 3.75-15 μg/ml for, Urtica dioica and Iris pseudacorus against gram-positive and gram-negative bacteria individually. Furthermore, the MBCs of herbal essences were 1.8-15 μg/ml for, Urtica dioica and 15-30 μg/ml for Iris.
Conclusion: The application of essential oils for the bio-control of diseases, as a novel emerging alternative to antimicrobial treatments, lead to safer and more environmental management for infective diseases.
References
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Copyright
© 2014, Author(s). This open-access article is available under the Creative Commons Attribution 4.0 (CC BY 4.0) International License (https://creativecommons.org/licenses/by/4.0/), which allows for unrestricted use, distribution, and reproduction in any medium, provided that the original work is properly cited.
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