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Zahedan Journal of Research in Medical Sciences
The Official Journal of Zahedan University of Medical Sciences
e-ISSN 2383-2894
Image Credit:Zahedan J Res Med Sci
Outline
Effect of Cytosolic Extract of Alternaria Alaternata Fungus on Monocyte-Derived Dendritic Cell Phagocytosis Ability and T-Lymphocyte Proliferation in the Presence of Myelin Basic Protein
Authors
1Department of Microbiology, Faculty of Veterinary Medicine, Urmia University, Urmia, Iran
*Corresponding Author: Department of Microbiology, Faculty of Veterinary Medicine, Urmia University, Urmia, Iran Email: alireza_trtr@yahoo.com
Published online:Jan 15, 2013
Article type:Research Article
Received:Jul 03, 2012
Accepted:Oct 20, 2012
How to Cite:Loghmani A, Delirezh N, Ownagh A, Mohebalian H. Effect of Cytosolic Extract of Alternaria Alaternata Fungus on Monocyte-Derived Dendritic Cell Phagocytosis Ability and T-Lymphocyte Proliferation in the Presence of Myelin Basic Protein. Zahedan J Res Med Sci. 2013;15(11):e92793. doi:
Abstract
Background: Multiple sclerosis (MS) is an autoimmune disease with impairment in function of CNS, meanwhile macrophages and dendritic cells (DC) can cause inflammation and damage to the myelin of nerve cells by releasing Reactive oxygen species (ROS) and other harmful substances when these cells get matured. We investigated the effect of Alternaria alternata (A. alternata) extract on phagocytic T cell stimulation activity of DC pulsed with Myelin Basic Protein (MBP) as a laboratory model of MS.
Materials and Methods: Plastic adherent monocytes were cultured with granulocyte-macrophage colony stimulating factor (GM-CSF), interleukin-4 for converting these cells to MoDc (Monocyte-Derived Dendritic Cell), pulsed with MBP, matured in the presence of monocyte-conditioned medium (MCM) in control group and MCM+Alternaria. alternata extract in treatment groups. Phagocytic activity of DC was evaluated and T cell responses were investigated by MTT test.
Results: Phagocytic activity in treatment groups decreased significantly in compare with control group. Meanwhile, DC couldn’t stimulate T cell proliferation.
Conclusion: A. alternata extract decreased phagocytic activity of MoDc-pulsed with MBP and had no effect on T cell proliferation may provide a new strategy on immunotraphy of multiple sclerosis.
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Copyright
© 2013, Author(s). This open-access article is available under the Creative Commons Attribution 4.0 (CC BY 4.0) International License (https://creativecommons.org/licenses/by/4.0/), which allows for unrestricted use, distribution, and reproduction in any medium, provided that the original work is properly cited.
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