A Comparison of Cytotoxic Effects of Mangifera Indica L. and Juglans RegiaAqueous Extract on Human Chronic Lymphocytic Leukemia

Abdollmajid Ayatollahi; Jalil Rahmati; Ahmad Salimi; Jalal Pourahmad

doi:10.22037/ijpr.2019.111977.13462

IJ Pharmaceutical Research

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https://doi.org/10.22037/ijpr.2019.111977.13462

A Comparison of Cytotoxic Effects of Mangifera Indica L. and Juglans RegiaAqueous Extract on Human Chronic Lymphocytic Leukemia

Author(s):

Abdollmajid Ayatollahi¹,

Jalil Rahmati²,

Ahmad Salimi^3,*,

Jalal Pourahmad^2,**

1School of Pharmacy, Phytochemistry Research Center, Shahid Beheshti University of Medical Sciences, Tehran, Iran

2Department of Pharmacology and Toxicology, School of Pharmacy, Shahid Beheshti University of Medical Sciences, Tehran, Iran

3Department of Pharmacology and Toxicology, School of Pharmacy, Ardabil University of Medical Sciences, Ardabil, Iran

Corresponding Authors:

IJ Pharmaceutical Research:Vol. 18, issue 4; 1843-1853

Published online:Sep 01, 2019

Article type:Research Article

Received:Jun 01, 2019

Accepted:Sep 01, 2019

How to Cite:Abdollmajid AyatollahiJalil RahmatiAhmad SalimiJalal PourahmadA Comparison of Cytotoxic Effects of Mangifera Indica L. and Juglans RegiaAqueous Extract on Human Chronic Lymphocytic Leukemia.Iran J Pharm Res.18(4):e127854.https://doi.org/10.22037/ijpr.2019.111977.13462.

Abstract

Natural products isolated from plant sources are well known for their pharmacological potential in diversity of disease treatments such as inflammatory or cancer conditions. Mango (Mangifera indica L.) and Juglans regia are thought to be rich of functional phytochemicals. To clarify the anticancer activity, aqueous extracts of Juglans regia (JR) nut and Mangifera indica L (MI) fruit were exanimated on chronic lymphocytic leukemia (CLL) B lymphocytes and their mitochondria and the results were compared with those of normal B lymphocytes. Cellular parameters such as viability and caspase 3 activity, and mitochondrial parameters such as reactive oxygen species (ROS), mitochondria membrane potential (MMP), mitochondrial swelling, and cytochrome c release were evaluated. Our results demonstrated that the extract of Mangifera indica L increased cytotoxicity and caspase 3 activation through mitochondria pathway only in CLL B lymphocytes and also the extract of Juglans regia did not show cytotoxicity and caspase 3 activation on CLL and healthy B lymphocytes. Our in-vitro findings on isolated mitochondria indicated that mitochondrial ROS formation, MMP collapse, and mitochondrial swelling and cytochrome c release were significantly (p < 0.05) increased after addition of Mangifera indica only in cancerous mitochondria. These results demonstrated that Mangifera indica can act as a promising source for anti-cancer drug candidates by directly and selectively targeting mitochondria and inducing selective mitochondria mediated apoptosis on CLL B lymphocytes.

Keywords

Chronic Lymphocytic Leukemia

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Author(s):

Abdollmajid Ayatollahi:[PubMed][Scholar]
Jalil Rahmati:[PubMed][Scholar]
Ahmad Salimi:[PubMed][Scholar]
Jalal Pourahmad:[PubMed][Scholar]