Prevalence of 𝛽-lactamases genes of IMP, SHV and PER in Pseudomonas aeruginosa isolated from hospitals in Kermanshah

Afsaneh  Salimi; Alisha Akya; Erfan  Haidari

Journal of Clinical Research in Paramedical Sciences

The Official Journal of Paramedical School, KUMS

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Prevalence of 𝛽-lactamases genes of IMP, SHV and PER in Pseudomonas aeruginosa isolated from hospitals in Kermanshah

Author(s):

Afsaneh Salimi¹,

Alisha Akya^2,*,

Erfan Haidari³

1. Student Research Committee, Kermanshah University of Medical Sciences, Kermanshah, Iran

2Nosocomial Infection Research Center, Kermanshah University of Medical Sciences, Kermanshah, Iran

3Department of Microbiology, Azad Univerisy of Sanandaj, Sanandaj, Iran

Journal of Clinical Research in Paramedical Sciences:Vol. 4, issue 2; e82001

Published online:Aug 13, 2015

Article type:Research Article

Received:Feb 14, 2015

Accepted:Jun 18, 2015

How to Cite: Salimi A, Akya A, Haidari E. Prevalence of 𝛽-lactamases genes of IMP, SHV and PER in Pseudomonas aeruginosa isolated from hospitals in Kermanshah.J Clin Res Paramed Sci.2015;4(2):e82001.

Abstract

Background: Pseudomonas aeruginosa is an opportunistic pathogen associated with nosocomial infections. The emergence and dissemination of metallo-𝛽-lactamases (MBLs) and extendedspectrum-𝛽-lactamases (ESBLs) have contributed to the high rate of
resistance among the p. aeruginosa isolates. This study investigated the prevalence of IMP, SHV and PER genes in p. aeruginosa isolates.
Methods: Antibiotic susceptibility testing was performed using the minimal inhibitory concentrations (MIC). Then the polymerase chain reaction (PCR) was used for the detection of 3 genes encoding IMP, SHV and PER.
Results: Of the 60 isolates tested, 38 (63.3%) were resistant to piperacilin, 42 (70%) to ceftazidime, 44(73.3%) to imipenem and 45 (75%) to cefepime. Five (8.3%) and 8(13.3%) of the isolates had PER and SHV genes by PCR results. In this study IMP gene was not found.
Conclusion: According to this study, P. aeruginosa isolates were highly resistant to antibiotics. Therefore the susceptibility of isolates should be tested before treatment. Among the three β-lactamases genes studied the highest prevalence was related to SHV gene. Given the clinical significance of MBLs and ESBLs producing isolates, identification of these organisms is essential in the hospitals in order to get a better therapeutic response and control of bacterial dissemination.

Keywords

Pseudomonas aeruginosa

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Author(s):

Afsaneh Salimi:[PubMed][Scholar]
Alisha Akya:[PubMed][Scholar]
Erfan Haidari:[PubMed][Scholar]