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Prevalence of 𝛽-lactamases genes of IMP, SHV and PER in Pseudomonas aeruginosa isolated from hospitals in Kermanshah

AUTHORS

avatar Afsaneh Salimi 1 , avatar Alisha Akya 2 , * , avatar Erfan Haidari 3

1 . Student Research Committee, Kermanshah University of Medical Sciences, Kermanshah, Iran

2 Nosocomial Infection Research Center, Kermanshah University of Medical Sciences, Kermanshah, Iran

3 Department of Microbiology, Azad Univerisy of Sanandaj, Sanandaj, Iran

How to Cite: Salimi A, Akya A, Haidari E . Prevalence of 𝛽-lactamases genes of IMP, SHV and PER in Pseudomonas aeruginosa isolated from hospitals in Kermanshah. J Clin Res Paramed Sci. 2015;4(2):e82001.

ARTICLE INFORMATION

Journal of Clinical Research in Paramedical Sciences: 4 (2); e82001
Published Online: August 13, 2015
Article Type: Research Article
Received: February 14, 2015
Accepted: June 18, 2015
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Abstract

Background: Pseudomonas aeruginosa is an opportunistic pathogen associated with nosocomial infections. The emergence and dissemination of metallo-𝛽-lactamases (MBLs) and extendedspectrum-𝛽-lactamases (ESBLs) have contributed to the high rate of
resistance among the p. aeruginosa isolates. This study investigated the prevalence of IMP, SHV and PER genes in p. aeruginosa isolates.
Methods: Antibiotic susceptibility testing was performed using the minimal inhibitory concentrations (MIC). Then the polymerase chain reaction (PCR) was used for the detection of 3 genes encoding IMP, SHV and PER.
Results: Of the 60 isolates tested, 38 (63.3%) were resistant to piperacilin, 42 (70%) to ceftazidime, 44(73.3%) to imipenem and 45 (75%) to cefepime. Five (8.3%) and 8(13.3%) of the isolates had PER and SHV genes by PCR results. In this study IMP gene was not found.
Conclusion: According to this study, P. aeruginosa isolates were highly resistant to antibiotics. Therefore the susceptibility of isolates should be tested before treatment. Among the three β-lactamases genes studied the highest prevalence was related to SHV gene. Given the clinical significance of MBLs and ESBLs producing isolates, identification of these organisms is essential in the hospitals in order to get a better therapeutic response and control of bacterial dissemination.

 

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© 2015, Journal of Clinical Research in Paramedical Sciences. This is an open-access article distributed under the terms of the Creative Commons Attribution-NonCommercial 4.0 International License (http://creativecommons.org/licenses/by-nc/4.0/) which permits copy and redistribute the material just in noncommercial usages, provided the original work is properly cited.
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