https://doi.org/10.5812/kowsar.20083645.2429

Identification of Candida Species Isolated From Oral Colonization in Iranian HIV-Positive Patients, by PCR-RFLP Method.

authors:

avatar Seyyed Amin Ayatollahi Mousavi 1 , * , avatar Samira Salari 2 , avatar Sasan Rezaie 3 , avatar Naser Shahabi Nejad 4 , avatar Sanaz Hadizadeh 4 , avatar Hossein Kamyabi 4 , avatar Hossein Aghasi 4

Department of Medical Mycology and Parasitology, School of Medicine, Medical University of Kerman, aminayatollahi@kmu.ac.ir, IR Iran
Department of Medical Mycology and Parasitology, School of Medicine, Medical University of Kerman, Kerman, IR Iran, and Mycology Research Center, Faculty of Veterinary Medicine, University of Tehran, Iran
Department of Medical Mycology and Parasitology, School of Public Health and Institute of Public Health Research, Tehran University of Medical Sciences, IR Iran
Kerman Research Center of Infectious and Tropical Medicine, IR Iran
Jundishapur Journal of Microbiology: Vol.5, issue 1; 336-340
article type: Research Article
received: March 1, 2011
accepted: May 10, 2011

how to cite: Mousavi S, Salari S, Rezaie S, Nejad N, Hadizadeh S, et al. Identification of Candida Species Isolated From Oral Colonization in Iranian HIV-Positive Patients, by PCR-RFLP Method.. Jundishapur J Microbiol. 2012;5(1): 336-340. https://doi.org/10.5812/kowsar.20083645.2429.

Abstract

Background: The incidence of opportunistic infections due to Candida albicans and other Candida spp. has been increasing. Rapid identification of candidiasis is important for the clinical management of immunocompromised patients. Polymerase chain reactionrestriction fragment length polymorphism (PCR-RFLP) is a rapid, sensitive, and specific method for detection of clinically important fungi.
Objectives: The purpose of this study was to identify Candida spp. isolated from the oral cavities of HIV-infected patients in southeastern Iran (Kerman), by using PCR-based restriction enzyme digestion.
Patients and Methods: We identified 96 Candida isolates obtained from 139 Iranian patients infected with the human  immunodeficiency virus (HIV), between April 2009 and April 2010, by using PCR-RFLP assay. Universal primers for the internal transcribed spacer (ITS) region (ITS1–ITS4) of the fungal rRNA genes were used for this assay.
Results: We successfully identified the different Candida spp. by using the restriction enzyme MspI. C. albicans was the most commonly identified species (82.2%), followed by C. glabrata (7.29%), C. parapsilosis and C. kefyr (both 4.1%), and C. tropicalis (2%).
Conclusions: PCR-RFLP is a highly sensitive, specific, and direct method for fungal detection and can be used for fungal epidemiological studies in HIV-positive and other immunocompromised patients.

  • Implication for health policy/practice/research/medical education:
    To have knowledge about the HIV positive patients and the role of Candida spp. and candidiasis in that group was so important forthe authors.
  • Please cite this paper as:
    Ayatollahi Mousavi SA, Salari S, Rezaie S, Shahabi Nejad N, Hadizadeh S, Kamyabi H. et al. Identification of Candida Species Isolated From Oral Colonization in Iranian HIV-Positive Patients, by PCR-RFLP Method. Jundishapur J Microbiol. 2012;5(1):336-40.
  • DOI:10.5812/kowsar.20083645.2429

© 2012, AJUMS. Published by Kowsar M.P.Co. All rights reserved.

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