The association between glutathione s-transfrases T1, M1, and P1 polymorphisms with end stage renal disease

Hamid Nomani; Ali Aidy; Asad Vaisi Raygani; Lida HaghNazari; Dariush Reissi; Hadi Mozafari

Journal of Kermanshah University of Medical Sciences

Peer-reviewed Medical Quarterly

Current Issue All Issues In Press Search Accepted Manuscripts

Journal Information Boards and Committees Indexing and Listing Sources Journal Metrics Publication Ethics and Malpractice Statement Reviewer and AE Registration Form Support Contact Us Open Peer Review (OPR)

Authors Guide Submit Manuscript

The association between glutathione s-transfrases T1, M1, and P1 polymorphisms with end stage renal disease

Author(s):

Hamid Nomani¹,

Ali Aidy^1,*,

Asad Vaisi Raygani¹,

Lida HaghNazari¹,

Dariush Reissi²,

Hadi Mozafari¹

1Dept. of Biochemistry, School of Medicine, Kermanshah University of Medical Sciences, Kermanshah, Iran

2Kidney Transplantation Center, Kermanshah University of Medical Sciences, Kermanshah, Iran

Journal of Kermanshah University of Medical Sciences:Vol. 17, issue 8; e74390

Published online:Nov 29, 2013

Article type:Research Article

Received:Apr 13, 2013

Accepted:Sep 10, 2013

How to Cite:Nomani H, Aidy A, Vaisi Raygani A, HaghNazari L, Reissi D, et al. The association between glutathione s-transfrases T1, M1, and P1 polymorphisms with end stage renal disease.J Kermanshah Univ Med Sci.2013;17(8):e74390.

Abstract

Background: End stage renal disease or ESRD is a progressive and irreversible deterioration in renal function in which the body loses ability to maintain metabolical balance, fluid and electrolytes. Glutathione s-transfrases (GSTs) are members of multigenic family which have essential role as an antioxidant in the cells. In this study, the polymorphism of GST, T1, M1 & P in ESRD patients were compared with those of control groups to determine the possible relationship between polymorphism of these enzymes and ESRD.

Methods: 136 of ESRD patients as experimental and 137 of healthy individuals as control group were examined. The GST T1 & M1 genotypes were determined in all individuals with multiplex- PCR and GST P1 polymorphism was determined with PCR-RFLP.

Results: The distribution of GSTT1& M1 of T1⁺-M1⁺(wild), T1⁺-M1¯, T1¯-M1⁺and T1¯-M1¯(null) genotypes in control group were 45(32.8%), 40(29.2%), 28(20.4%) and 24(17.5%) and in experimental group were17(12.8%), 46(34.6%), 21(15.8%) and 49(36.8%) respectively (P<0.001).

Conclusion: As the reduction of GST T1⁺-M1⁺ (wild) was observed with more frequency in patients with ESRD compared to control group (12.8%vs.32.8%), this genotype can be associated with the risk of ESRD.

Keywords

GST

ESRD

PCR-RFLP

Fulltext

References

1.
The references of this article is available on PDF.

comments