Electroreduction of Tacrolimus as Immunosuppressant agent at Hanging Mercury Drop Electrode: Application to Determination in Pharmaceutical Formulation

authors:

avatar Mohammadbagher Majnooni 1 , 2 , avatar Mohammad B. Gholivand 3 , * , avatar Mehdi Khodadadian 3 , avatar Nazanin-Sadat Afnanzade 2 , avatar Gholamreza Bahrami 1 , 2

Medical Biology Research Center, Kermanshah University of Medical Science, Kermanshah, Iran
School of Pharmacy, Kermanshah University of Medical Science, Kermanshah, Iran
Department of Chemistry, Faculty of Science, Razi University, Kermanshah, Iran
Journal of Reports in Pharmaceutical Sciences: Vol.1, issue 1; 41-48
published online: March 28, 2012
article type: Research Article
received: February 28, 2012
revised: March 12, 2012
accepted: March 19, 2012

how to cite: Majnooni M, Gholivand M B, Khodadadian M, Afnanzade N, Bahrami G. Electroreduction of Tacrolimus as Immunosuppressant agent at Hanging Mercury Drop Electrode: Application to Determination in Pharmaceutical Formulation. J Rep Pharm Sci. 2012;1(1):e147802. 

Abstract

The adsorptive behavior of the immunosuppressive agent tacrolimus was studied by cyclic and differential-pulse voltammetry on a hanging mercury drop electrode (HMDE). The drug was accumulated at HMDE and two well-defined peak currents were obtained at -1353 and -1417 mV vs. SCE (saturated KCl) in borate buffer (pH 10.0) + 0.1 KCl solution. A voltammetric procedure was developed for the determination of tacrolimus using differential-pulse adsorptive stripping voltammetry (DPAdSV). The optimum working conditions for determination of the drug were established. The analysis of tacrolimus in pharmaceutical dosage forms was carried out satisfactorily.