Relationship between synthesis of P21 and Cyclin D1 proteins and histomorphometric parameters of mice testicular tissue in presence of Aflatoxin G1

authors:

avatar Cyrus Jalili , avatar Toraj Zamir-Nasta , avatar laya ghahari , * , avatar Seyran Kakeh baraei

Koomesh: Vol.21, issue 1; 195-200
published online: March 01, 2019
article type: Research Article
received: December 03, 2017
accepted: July 01, 2018

how to cite: Jalili C, Zamir-Nasta T, ghahari L, Kakeh baraei S. Relationship between synthesis of P21 and Cyclin D1 proteins and histomorphometric parameters of mice testicular tissue in presence of Aflatoxin G1. koomesh. 2019;21(1):e153057. 

Abstract

Introduction: AFG1 after B1 is the most important aflatoxin found in beans and grains.  Remarkably, aflatoxin cause a decrease in production and quality of sperm. In the current study, the effect of AFG1 on spermatogenesis, spermiogenicity, synthesis of P21 and cyclin D1 proteins in testicular tissue was investigated. Materials and Methods: 24 male albino mice were divided into 4 groups control group intraperitoneally received 0.2 ml corn oil and experimental groups received AFG1 (20 μg) for 7, 15, and 35 days, respectively. After 7, 15 and 35 days, the testicular tissue samples were taken, and after preparing tissue sections, hematoxylin and eosin (H;E) staining was performed. Histomorphometric parameters and the immunohistochemical analysis of proteins of P21 and cyclin D1 were investigated. Data analysis was carried out using one-way ANOVA at the significance level of p

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