Logo
homeHome
toggle_offCompany ProfilegroupsBoards & Staffsworkspace_premiumMemberships & PartnersschoolAcademy of Journalismperson_addCareerslinkBlog(EN)emailContact Us
list_altOur JournalslightbulbInstruction for AuthorsbookmarkBrieflands PolicieshandshakePublish My Researchplay_circleJournal Management Systemcredit_cardArticle Processing Chargeszoom_inOpen Peer ReviewimagePublishing Services
menu_bookPublish My Book

Archives of Clinical Infectious Diseases

Infectious Diseases and Tropical Medicine Research Center, SBUMS

homeHome
play_arrowCurrent Issuecheck_circleAccepted ManuscriptslistAll Issuesformat_indent_increaseIn PresssearchSearch
settingsInstructions
bookmarkJournal InformationgroupsEditors & BoardsshareIndexing and Listing Sourcesshow_chartJournal MetricsbalancePublication Ethics and Malpractice StatementemailSupportphoneContact Usassignment_indReviewer and AE Registration Formzoom_inOpen Peer Review (OPR)
format_list_bulletedAPC
Authors GuideSubmit Manuscript

Outlines

Cloning and Expression of Helicobacter Pylori UreB122(a Segment of the B-subunit of Urease Gene)

Author(s):
Shahin Najar PeerayehShahin Najar Peerayeh1,*, Moein FarshchianMoein Farshchian1, Majid SadeghizadehMajid Sadeghizadeh2, Javad AtoofiJavad Atoofi1
1Department of Microbiology, Faculty of Medical Sciences, Tarbiat Modares University, Tehran, IR Iran
2Department of Genetic, School of Basic Sciences, Tarbiat Modares University, Tehran, IR Iran
Archives of Clinical Infectious Diseases:Vol. 6, issue 4; 161-4
Published online:Oct 31, 2011
Article type:Research Article
How to Cite:Najar Peerayeh S, Farshchian M, Sadeghizadeh M, Atoofi J. Cloning and Expression of Helicobacter Pylori UreB122(a Segment of the B-subunit of Urease Gene). Arch Clin Infect Dis. 2011;6(4):. doi:

Abstract

Introduction:

Helicobacter pylori is associated with the chronic gastritis, peptic ulcer, gastric adenocarcinoma, and gastric mucosa-associated lymphoid tissue (MALT) lymphoma. Moreover, antibiotic therapies do not protect against potential re-infection while have risk for development of drug resistance. Therefore, vaccine-mediated protection against H. pylori became an attractive clinical interest. H. pylori urease plays an important role in survival and pathogenesis of the infection. In this study the UreB122 (aa143-264) gene cloned in pET expression vector and the recombinant protein (rUreB122) was over expressed in Escherichia coli (E. coli).

Patients and Methods:

Genomic DNA of the standard H. pylori strain 26695 was isolated as the template and UreB122 gene was amplified by PCR. Prokaryote expression vector pET32a was inserted with UreB122 gene (pET32a-UreB122). The recombinant plasmid was used to transform competent E. coli DH5?, and positive clones were selected. Then the recombinant plasmid was used to transform E. coli BL21DE3 for expression of recombinant protein UreB122.The expression of recombinant protein was induced by isopropythio-?-D-galctoside (IPTG) at different concentration

Results:

In comparison with the reported corresponding sequences, the nucleotide sequence homology of UreB122 gene was 99.9%. UreB122 fusion protein was able to react with the anti His-Tag antibody .

Keywords
Helicobacter Pylori
Urease
Recombinant Protein
pET32a.

Full Text

Full text is available in PDF
Import into EndNoteImport into BibTex
Share on
Comments
Number of Comments:0
Cited by
Metrics

Purchasing Reprints

  • Copyright Clearance Center (CCC) handles bulk orders for article reprints for Brieflands. To place an order for reprints, please click here (   https://www.copyright.com/landing/reprintsinquiryform/ ). Clicking this link will bring you to a CCC request form where you can provide the details of your order. Once complete, please click the ‘Submit Request’ button and CCC’s Reprints Services team will generate a quote for your review.
Search Relations

Author(s):

Related Articles

Related Article in PubMed

Related Article in Google Scholar

Create Citation Alert via Google Reader