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https://doi.org/10.5812/archcid.18690

Frequency of bla TEM, bla SHV, bla CTX-M, and qnrA Among Escherichia coli Isolated From Urinary Tract Infection

Author(s):
Shima AbdiShima Abdi1, Reza RanjbarReza Ranjbar2, Mojdeh Hakemi ValaMojdeh Hakemi Vala3,*, Nematollah JonaidiNematollah Jonaidi4, Ozra Baghery BejestanyOzra Baghery Bejestany2, Fatemeh Baghery BejestanyFatemeh Baghery Bejestany1
1Department of Microbiology, Pharmaceutical Sciences Branch, Islamic Azad University, Tehran, IR Iran
2Molecular Biology Research Center, Baqiyatallah University of Medical Sciences, Tehran, IR Iran
3Department of Microbiology, Shahid Beheshti University of Medical Sciences, Tehran, IR Iran
4Health Research Center, Baqiyatallah University of Medical Sciences, Tehran, IR Iran
*Corresponding Author: Department of Microbiology, Medical School, Shahid Beheshti University of Medical Sciences, Tehran, IR Iran. Tel: +98-2123872556, Fax: +98-2122439964, [email protected]. Email: [email protected]
Archives of Clinical Infectious Diseases:Vol. 9, issue 1; 18690
Published online:Jan 24, 2014
Article type:Research Article
Received:Mar 01, 2014
Accepted:May 09, 2014
How to Cite:Abdi S, Ranjbar R, Hakemi Vala M, Jonaidi N, Baghery Bejestany O, et al. Frequency of bla TEM, bla SHV, bla CTX-M, and qnrA Among Escherichia coli Isolated From Urinary Tract Infection. Arch Clin Infect Dis. 2014;9(1):18690. doi: https://doi.org/10.5812/archcid.18690

Abstract

Background:

Escherichia coli is the most important as well as the most common bacteria causing urinary tract infections (UTIs) and its resistance to common antibiotics is increasing. Extended-spectrum beta-lactamase (ESBL) producer E. coli strains can resist against the third-generation and fourth-generation cephalosporins.

Objectives:

This study aimed to evaluate the resistance profile of E. coli isolated from patients with UTIs referred to Imam Khomeini and Baqiyatallah Hospitals, Tehran, Iran, through phenotypic and molecular methods.

Materials and Methods:

During 2010-2011, 180 urine samples of patients with UTIs from Imam Khomeini and Baqiyatallah Hospitals were collected. Based on the standard bacteriologic tests, E. coli isolates were identified. Resistance to common antibiotics was tested by the Kirby-Bauer method and reconfirmed by determining minimum inhibitory concentration (MIC) through microdilution method. Further phenotypic double-disk synergy test (DDST) was performed to screen the ESBL producer strains. Resistance genes related to ESBL and qnrA were evaluated by Polymerase chain Reaction (PCR).

Results:

A total of 100 E. coli strains were examined by antibiogram and the rates of resistance to the tested antibiotics were as follows: 100% to penicillin and amoxicillin, 77% to amoxicillin-clavulanic acid, 72% to ceftazidime, 69% to cefotaxime, 47% to cefoxitin, 46% to ceftriaxone, 43% to cephalexin, 27% to aztreonam, 53% to nalidixic acid, 51% to ciprofloxacin, and 2% to imipenem. The MIC to ciprofloxacin, cefazolin, and ceftriaxone were ? 0.249, ? 0.508, and ? 0.044, respectively. Moreover, 20% of E. coli isolates were ESBL-producing isolates by DDST. The frequency of bla CTX-M, bla TEM, bla SHV, and qnrA genes was 87%, 82%, 65%, and 39%, respectively.

Conclusions:

Considering the high prevalence of ESBL genes (bla CTX-M, 87%; and bla TEM, 82%), fluoroquinolones may be used as an alternative drug in treatment, although resistance to this family is increasing as well. As a result, this increasing trend should be prevented using appropriate guidelines for prescription.

Keywords
Escherichia coli
bla CTX-M
bla TEM
beta-Lactamases
bla SHV

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Copyright

© 2014, Author(s). This open-access article is available under the Creative Commons Attribution 4.0 (CC BY 4.0) International License (https://creativecommons.org/licenses/by/4.0/), which allows for unrestricted use, distribution, and reproduction in any medium, provided that the original work is properly cited.

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