Archives of Clinical Infectious Diseases
Rasool Estakhri1,*, 
Homayoon Dolatkhah2, Ardavan Ghazanchaei3, Behrooz Pourasgari4, Manuchehr Nourazarian4Helicobacter pylori is known as an agent which may involve in the occurrence of peptic ulcer, gastric cancer and also other known and unknown diseases. Treatment of the infection with antibiotics eradicates the disease and prevents its pathologic effects. A noninvasive and inexpensive method for detection of the infection is needed. In this study the diagnostic values of serum and saliva anti H. pylori IgG was evaluated.
The saliva and blood samples were collected from 114 patients who underwent upper GI endoscopy and gastric biopsy. Tissue samples were examined by rapid urease test and microscopic study. Saliva and serum samples were tested by ELISA-based test for anti H. pylori IgG, using a commercial kit.
From 114 cases, 61(53.5%) patients were positive for H. pylori in rapid urease test and microscopic study and 53(46.5%) were negative in both tests. Rates of positive result for H. pylori in patients with and without peptic ulcer were almost similar. Mean values of anti H. pylori IgG in saliva and serum of H. pylori positive patients were higher than H. pylori negative patients. Sensitivity, specificity, positive predictive value, negative predictive value and accuracy of tests in saliva were 83.6%, 71.7%, 77.3%, 79.1%, 78.1% and in serum were 90.2%, 86.8%, 88.7%, 88.4% and 88.6% respectively.
It was concluded that ELISA-based anti H. pylori IgG test in saliva could be used as an alternative diagnostic test in the absence of other invasive procedures.
© 2008, Author(s). This open-access article is available under the Creative Commons Attribution 4.0 (CC BY 4.0) International License (https://creativecommons.org/licenses/by/4.0/), which allows for unrestricted use, distribution, and reproduction in any medium, provided that the original work is properly cited.
Rostami F, Javdani Shahedin G, Bashizadeh-Fakhar H, Divsalar S, Jalalian M. Diagnostic Accuracy of Enzyme-Linked Immunosorbent Assay Compared with Polymerase Chain Reaction in Diagnosing Helicobacter pylori in Patients with Gastric Problems. Arch Clin Infect Dis. 2024;19(6):e150644. doi: https://doi.org/10.5812/archcid-150644
Imanieh MH, Dehghani SM, Masjedi A, Rezaianzadeh A, Haghighat M. The Role of Serological Tests in Diagnosis of Helicobacter pylori Infection in Children. J Compr Ped. 2014;5(3):e17422. doi: https://doi.org/10.17795/compreped-17422
Fabricio Guaman J, Bayas-Morejon IF, Arcos V, Tigre-Leon A, Lucio-Quintana A, et al. Detection of Helicobacter pylori from Human Biological Samples (Feces) by Antigenic Screening and Culture. Jundishapur J Microbiol. 2018;11(7):e66721. doi: https://doi.org/10.5812/jjm.66721
Falsafi T, Lavasani P, Basardeh I, Massarrat S, Landarani Z. Evaluation of an Iranian Home-made Helicobacter pylori Stool Antigen ELISA Kit. Jundishapur J Microbiol. 2014;7(6):e10629. doi: https://doi.org/10.5812/jjm.10629
Salimi M, Sohrabi MB, Zolfaghari P, Mirghasemi M, Yahyaei E, et al. Comparison of diagnostic accuracy of serologic test with histology for Helicobacter pylori in diabetic patients with dyspepsia. J Inflamm Dis. 2024;19(6):e155938. doi:
Ordering Reprints
Articles are published under the Creative Commons license stated on each article. No permission or royalty fee is required for uses permitted by that license. CCC handles optional bulk and customized reprint orders. Any quotation covers production and delivery services only, not copyright permission. > Request Reprints from CCCÂ
Author(s):
