This article has been retracted at the request of the authors of this paper.
Background:
Tissue engineering is the science of tissue design and one of the main branches of regenerative medicine that aims to improve and repair tissue injuries.
The Official Journal of Zahedan University of Medical Sciences
e-ISSN 2345-6841
p-ISSN 2345-6833
Retracted Article: Preparation of Decellularized Bovine Tendon Scaffold and Evaluation of Its Interaction with Adipose Tissue-Derived Mesenchymal Stem Cells
Authors
1Department of Engineering Sciences, Faculty of Advanced Technologies, University of Mohaghegh Ardabili, Namin, Iran
2Bio Science and Biotechnology Research Center (BBRC), Sabalan University of Advanced Technologies (SUAT), Namin, Iran
3Department of Physiology and Medical Physics, Faculty of Medicine, University of Al-Ameed, Karbala, Iraq
4Department of Biology, Faculty of Science, University of Mohaghegh Ardabili, Ardabil, Iran
*Corresponding Author: Department of Engineering Sciences, Faculty of Advanced Technologies, University of Mohaghegh Ardabili, Namin, Iran. Email: abdolmalekiarash1364@gmail.com
Published online:May 09, 2020
Article type:Research Article
Received:Feb 08, 2020
Accepted:Feb 22, 2020
How to Cite:Abdolmaleki A, A. Ghanimi H, Asadi A, Taghizadeh-Momen L. Retracted Article: Preparation of Decellularized Bovine Tendon Scaffold and Evaluation of Its Interaction with Adipose Tissue-Derived Mesenchymal Stem Cells. Gene Cell Tissue. 2020;7(2):e101735. doi: https://doi.org/10.5812/gct.101735
Abstract
Objectives:
This study aimed to decellularize the tissue of bovine Achilles tendon and create a natural 3D scaffold. Then, the interaction of human adipose tissue-derived mesenchymal stem cells (hAd-MSCs) with this 3D scaffold was evaluated for use in tendon injuries.
Methods:
The bovine Achilles tendon was obtained from a slaughterhouse and decellularized by the combination of ethylenediaminetetraacetic acid (EDTA) and sodium dodecyl sulfate (SDS). Histological and biomechanical tests were used to evaluate the quality of decellularized scaffolds. Adipose-derived mesenchymal stem cells were cultured on scaffolds, and cell viability and cell behavior were evaluated by the MTT test and scanning electron microscopy.
Results:
The results of histological and biomechanical tests showed the complete removal of cells with the preservation of the extracellular matrix. The results of cell culture on scaffolds also showed that optical absorption in scaffolds containing cells increased over time.
Conclusions:
In general, the decellularized scaffold in this study did not undergo significant structural changes in the tendon tissue. The interaction between hAd-MSCs and the decellularized scaffold revealed that the scaffold was somehow suitable for cell culture. However, it needs to be more investigated for use in the treatment of tendon injuries of the athletes.
Copyright
Copyright © 2020, Gene, Cell and Tissue. This is an open-access article distributed under the terms of the Creative Commons Attribution-NonCommercial 4.0 International License (http://creativecommons.org/licenses/by-nc/4.0/) which permits copy and redistribute the material just in noncommercial usages, provided the original work is properly cited.
Similar Articles
17
Aug
2020
Retraction Note: Preparation of Decellularized Bovine Tendon Scaffold and Evaluation of Its Interaction with Adipose Tissue-Derived Mesenchymal Stem Cells
Arash Abdolmaleki,
Hussein A. Ghanimi,
Asadollah Asadi,
Leila Taghizadeh-Momen
Abdolmaleki A, A. Ghanimi H, Asadi A, Taghizadeh-Momen L. Retraction Note: Preparation of Decellularized Bovine Tendon Scaffold and Evaluation of Its Interaction with Adipose Tissue-Derived Mesenchymal Stem Cells. Gene Cell Tissue. 2020;7(4):e108641. doi: https://doi.org/10.5812/gct.108641
31
Jul
2021
Investigation and Comparison of the Effect of TGF-β3, kartogenin and Avocado/Soybean Unsaponifiables on the In-vitro and In-vivo Chondrogenesis of Human Adipose-Derived Stem Cells on Fibrin Scaffold
Batool Hashemibeni,
Mohammad Ali Izadi,
Ali Valiani,
Ibrahim Esfandiari,
Hamid Bahramian,
Hengameh Dortaj
,et al.Hashemibeni B, Izadi MA, Valiani A, Esfandiari I, Bahramian H, et al. Investigation and Comparison of the Effect of TGF-β3, kartogenin and Avocado/Soybean Unsaponifiables on the In-vitro and In-vivo Chondrogenesis of Human Adipose-Derived Stem Cells on Fibrin Scaffold. Iran J Pharm Res. 2021;20(3):e124296. doi: https://doi.org/10.22037/ijpr.2020.114420.14851
10
Mar
2024
Reconstruction of Trachea Defect by Composite of PLGA/FG Materials with Differentiated Human Adipose-Derived Stem Cells in a Dog Model
Seyed Khalil Pestehei,
Mahdieh Ghiasi
Pestehei SK, Ghiasi M. Reconstruction of Trachea Defect by Composite of PLGA/FG Materials with Differentiated Human Adipose-Derived Stem Cells in a Dog Model. Arch Neurosci. 2024;11(2):e143723. doi: https://doi.org/10.5812/ans-143723
9
Jan
2024
Evaluating the Biocompatibility of Decellularized Pancreas and Its Histological Features as a Support Structure for Adipose Mesenchymal Stem Cell Culture
Neda Farazi,
Arash Abdolmaleki,
Asadollah Asadi,
Saber Zahri
Farazi N, Abdolmaleki A, Asadi A, Zahri S. Evaluating the Biocompatibility of Decellularized Pancreas and Its Histological Features as a Support Structure for Adipose Mesenchymal Stem Cell Culture. Gene Cell Tissue. 2024;11(2):e139054. doi: https://doi.org/10.5812/gct-139054
22
Oct
2024
Evaluation the Impressionability of Acellular Scaffolds in Presence of Different Combination of Umbilical Cord Blood Stem Cells and Platelet Rich Fibrin in Repair of Knee Defects in Rabbit (Novel Method with Xeno-Material Elements
Seyed Khalil Pestehei,
Mahdieh Ghiasi,
Nima Bagheri
Pestehei SK, Ghiasi M, Bagheri N. Evaluation the Impressionability of Acellular Scaffolds in Presence of Different Combination of Umbilical Cord Blood Stem Cells and Platelet Rich Fibrin in Repair of Knee Defects in Rabbit (Novel Method with Xeno-Material Elements. Arch Neurosci. 2024;11(4):e147989. doi: https://doi.org/10.5812/ans-147989
Cited by
- Scopus by DOI: 0
Last Update: 1 month ago
- Scopus by Title: 0
Last Update: 1 month ago
- Scopus by Title (Ref): 0
Last Update: 1 month ago
- CrossRef: 0
Last Update: 5 hours ago
- OpenAlex: 1
Last Update: 2 days ago
- OpenCitations: 0
Last Update: 4 days ago
Comments
Number of Comments:0