Clinical values of AFP, GPC3 mRNA in peripheral blood for prediction of hepatocellular carcinoma recurrence following OLT

Yuliang Wang; Zhongyang Shen; Zhijun Zhu; Ruifa Han; Mingsheng Huai

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Clinical values of AFP, GPC3 mRNA in peripheral blood for prediction of hepatocellular carcinoma recurrence following OLT

Author(s):

Yuliang Wang¹,

Zhongyang Shen^1,*,

Zhijun Zhu¹,

Ruifa Han²,

Mingsheng Huai¹

1Orient Organ Transplant Center, First Central Clinic Institute of Tianjin Medical University, Key Lab for Critical Care Medicine of the Ministry of Health, Tianjin, China

2Tianjin Institute of Urology, Tianjin Medical University, Tianjin, China

Hepatitis Monthly:Vol. 11, issue 3; e170028

Published online:May 30, 2011

Article type:Research Article

The editors have expressed concern about this article. See Expression of Concern for Clinical Values of AFP, GPC3 mRNA in Peripheral Blood for Prediction of Hepatocellular Carcinoma Recurrence Following OLT [Hepat Mon. 2011; 11 (3): e170028].

How to Cite:Wang Y, Shen Z, Zhu Z, Han R, Huai M. Clinical values of AFP, GPC3 mRNA in peripheral blood for prediction of hepatocellular carcinoma recurrence following OLT.Hepat Mon.2011;11(3):e170028.

Abstract

Background: Hepatocellular carcinoma (HCC) is one of the most common malignancies worldwide. Annually, about 200,000 patients died of HCC in China. Liver transplantation (LT) holds great theoretical appeal in treating HCC. However, the high recurrence rate after transplantation is the most important limiting factor for long-term survival.

Objectives: To assess the value of alpha-fetoprotein (AFP) messenger RNA (mRNA), Glypican-3 (GPC3) mRNA-expressing cells in the peripheral blood (PB) for prediction of HCC recurrence following orthotopic liver transplantation (OLT).

Patients and Methods: 29 patients with HCC who underwent OLT with a minimum clinical follow-up of 12 months were included in this retrospective study. We detected AFP mRNA, GPC3 mRNA-expressing cells in the PB by TaqMan real-time reverse transcriptase-polymerase chain reaction (RT-PCR), pre-, intra- and post-operatively. The early recurrence of patients was evaluated.

Results: 8 (28%), 15 (52%), and 9 (31%) patients had AFP mRNA detected pre-, intra-, and postoperatively, respectively. With 12 months of follow-up, HCC recurred in 7 (24%) patients. Univariate analysis revealed that positive pre- and post-operative AFP mRNA, TNM stage as well as vascular invasion were significant predictors for the HCC recurrence. Multivariate analysis revealed that being positive for AFP mRNA pre-operatively remained a significant risk factor for HCC recurrence after OLT. GPC3 mRNA was expressed in all PB samples. There was no significant difference in the expression levels of GPC3 mRNA between the HCC and control groups. There were no significant differences in GPC3 mRNA expression values between those patients with and without tumor recurrence.

Conclusions: The pre-operative detection of circulating AFP mRNA-expressing cells could be a useful predictor for HCC recurrence following OLT. GPC3 mRNA-expressing cells in PB seem to have no diagnostic value.

Keywords

Liver Transplantation

Hepatocellular carcinoma

Alpha-fetoprotein

Glypican-3

Recurrence

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Author(s):

Yuliang Wang:[PubMed][Scholar]
Zhongyang Shen:[PubMed][Scholar]
Zhijun Zhu:[PubMed][Scholar]
Ruifa Han:[PubMed][Scholar]
Mingsheng Huai:[PubMed][Scholar]

Official Journal of Research Center for Gastroenterology and Liver Diseases

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Clinical values of AFP, GPC3 mRNA in peripheral blood for prediction of hepatocellular carcinoma recurrence following OLT

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