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Official Journal of Research Center for Gastroenterology and Liver Diseases
e-ISSN 1735-3408
p-ISSN 1735-143X
Seroepidemiology and Molecular Characterization of Hepatitis E Virus in Macaca Mulatta from a Village in Yunnan, China, where Infection with this Virus Is Endemic
Authors
1Faculty of Life Science and Technology, Kunming University of Science and Technology, China
2Zoonosis Laboratory, School of Agriculture and Biology, Shanghai Jiao Tong University, China
3Institute of Medical Biology, Chinese Academy of Medical Sciences and Peking Union Medical College, hzl@imbcams.com, China
*Corresponding Author: Institute of Medical Biology, Chinese Academy of Medical Sciences and Peking Union Medical College, hzl@imbcams.com, China. Tel: +86-8718403316. Fax: +86-8718403245. Email: hzl@imbcams.com
Published online:Sep 30, 2011
Article type:Research Article
Received:Apr 14, 2011
Accepted:Jul 18, 2011
How to Cite:Huang F, Yu W, Hua X, Jing S, Zeng W, et al. Seroepidemiology and Molecular Characterization of Hepatitis E Virus in Macaca Mulatta from a Village in Yunnan, China, where Infection with this Virus Is Endemic. Hepat Mon. 2011;11(9):. doi: https://doi.org/10.5812/kowsar.1735143X.730
Abstract
Background: Hepatitis E virus (HEV) infection is a significant public health concern and has been identified as a zoonotic infection.
Objectives: Since no reports have characterized the epidemiological and genotypicfeatures of HEV infections in Macaca mulatta (rhesus macaques) from Yunnan, China, where swine HEV infections are endemic, we aimed to investigate these characteristics.
Materials and Methods: Seroepidemiological and molecular characterization of HEV in both Macaca mulatta and pigs from the Yunnan province of China were conducted using enzyme-linked immunosorbent assay (ELISA) and reverse transcription-nested PCR (RT-nPCR). Four hundred and eighty-two stool samples (320 from Macaca mulatta and 162 from pigs) and 92 serum samples (all from Macaca mulatta) were collected for the detection of HEV RNA and anti-HEV antibodies (IgG/IgM).
Results: Thirty-three rhesus macaques (35.87%) were positive for HEV IgG. Of these, 3 were also positive for HEV IgM. Four different strains of swine HEV RNA were detected in pigs; however, we failed to detect any in Macaca mulatta.
Conclusions: Results indicate that Macaca mulatta may not be a natural reservoir of HEV.
Objectives: Since no reports have characterized the epidemiological and genotypicfeatures of HEV infections in Macaca mulatta (rhesus macaques) from Yunnan, China, where swine HEV infections are endemic, we aimed to investigate these characteristics.
Materials and Methods: Seroepidemiological and molecular characterization of HEV in both Macaca mulatta and pigs from the Yunnan province of China were conducted using enzyme-linked immunosorbent assay (ELISA) and reverse transcription-nested PCR (RT-nPCR). Four hundred and eighty-two stool samples (320 from Macaca mulatta and 162 from pigs) and 92 serum samples (all from Macaca mulatta) were collected for the detection of HEV RNA and anti-HEV antibodies (IgG/IgM).
Results: Thirty-three rhesus macaques (35.87%) were positive for HEV IgG. Of these, 3 were also positive for HEV IgM. Four different strains of swine HEV RNA were detected in pigs; however, we failed to detect any in Macaca mulatta.
Conclusions: Results indicate that Macaca mulatta may not be a natural reservoir of HEV.
- Implication for health policy/practice/research/medical education:
Macaca mulatta are often used as HEV model to evaluate HEV vaccine, pathogenesis and xenotransplantation. This study was designed to investigate if Macaca mulatta can be natural host of the virus in area where HEV infection is endemic.
-
Please cite this paper as:
Huang F, Yu W, Hua X, Jing S, Zeng W, He Z. Seroepidemiology and Molecular Characterization of Hepatitis E Virus in Macaca Mulatta from a Village in Yunnan, China, where Infection with this Virus Is Endemic. Hepat Mon. 2011;11(9):745-9. DOI: 10.5812/ kowsar.1735143X.730
Copyright© 2011 Kowsar M.P.Co. All rights reserved.
Highlights
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© 2011, Author(s). This open-access article is available under the Creative Commons Attribution 4.0 (CC BY 4.0) International License (https://creativecommons.org/licenses/by/4.0/), which allows for unrestricted use, distribution, and reproduction in any medium, provided that the original work is properly cited.
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