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Hepatitis Monthly
Official Journal of Research Center for Gastroenterology and Liver Diseases
The effect of desacetyluvaricin on the expression of TLR4 and P53 protein in Hepg 2.2.15
Author(s):
Bin Yu, Hai Bin He1, Xian Lin Wu2, Kang Li Liu2, Guang Xiong Zhou3, Guo Qiang Qian2, Da Hong Ju4, Xiao Yin Chen#2, Xiao Chen#5,*1Department of Gastroenterology, the People’s Hospital of Heshan, Guangdong, China
2Department of Traditional Chinese Medicine, Medicine College of Jinan University, Guangzhou, China
3Pharmaceutical College of Jinan University, Guangzhou, China
4Institute of Basic Theories of Traditional Chinese Medicine, China Academy of Traditional Chinese Medicine, Beijing, China
5Shusen Zheng, Department of Hepatobiliary and Pancreatic Surgery, First Affiliated Hospital, Hangzhou, China. Tel: +86- 57187236570, Fax: +86-57187236466. E-mail: [email protected]
*Corresponding Author: Xiao Yin Chen, Department of Traditional Chinese Medicine, Medicine College of Jinan University, No. 601, Huangpu West Road, Guangzhou, China. Tel: +861-3822263950, Fax: +86-2085221343. E-mail: [email protected] Email: [email protected]
# These authors have contributed equally.
Published online:May 31, 2011
Article type:Research Article
Received:Aug 29, 2010
Accepted:Dec 04, 2010
How to Cite:Yu B, He HB, Wu XL, Liu KL, Zhou GX, et al. The effect of desacetyluvaricin on the expression of TLR4 and P53 protein in Hepg 2.2.15. Hepat Mon. 2011;11(5):. doi:
Abstract
Background: Previous studies suggest that annonaceous may cause permeability glycoprotein (P-gp) function to abate, leading to cell apoptosis. It has also been reported that annonaceous acetogenins affect hepatocellular carcinoma (HCC) cells in the G1 phase, leading to apoptosis. Desacetyluvaricin (Des), a new type of annonaceous acetogenin monomer, has a significant effect on HCC, with few side effects.
Objectives: To investigate the effect of Des on the expression of Toll-like receptor 4 (TLR4) and P53 protein in HCC.
Materials and Methods: HCC HepG2.2.15 cell was cultured by routine method. HepG2.2.15 cells were divided into three groups: control group, treated with Des and DDP (cisplatin) which were examined by immunofluorescence flow cytometry for expression of TLR4 and P53.
Results: TLR4 was expressed by more cells in the Des group than in the cisplatin or serum-only groups (71.94%, 42.64%, and 37.16%, respectively; Des vs.cisplatin: p < 0.05; Des vs. serum only: p < 0.05), with no difference between the cisplatin and serum-only groups (p > 0.05). P53 was expressed by more cells in the Des and cisplatin groups than in the serum-only group (32.6%, 31.5% and 3.3%, respectively; Des vs. serum only, p < 0.05; cisplatin vs. serum only, p < 0.05), with no difference between the Des and cisplatin groups (p > 0.05).
Conclusions: Des increases TLR4 and P53 expression in HCC cells. Improved immune recognition by the former effect and induction of apoptosis by the latter could be the mechanisms of Des's clinical effects on HCC.
Keywords: Desacetyluvaricin; TLR4 receptor; P53 tumor suppressor protein; Flow cytometry; Hepatocellular carcinoma
2011 Kowsar M.P.Co. All rights reserved.
Copyright
© 2011, Author(s). This open-access article is available under the Creative Commons Attribution 4.0 (CC BY 4.0) International License (https://creativecommons.org/licenses/by/4.0/), which allows for unrestricted use, distribution, and reproduction in any medium, provided that the original work is properly cited.
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